川崎病患者粪便中多种超抗原基因的检测
Detection of multiple superantigen genes in stools of patients with Kawasaki disease.
作者信息
Suenaga Tomohiro, Suzuki Hiroyuki, Shibuta Shoichi, Takeuchi Takashi, Yoshikawa Norishige
机构信息
Department of Pediatrics, Wakayama Medical University, Wakayama, Japan.
出版信息
J Pediatr. 2009 Aug;155(2):266-70. doi: 10.1016/j.jpeds.2009.03.013. Epub 2009 May 15.
OBJECTIVES
To investigate whether superantigens (SAgs) are involved in the development of Kawasaki disease (KD) by examining SAg genes in the stool of patients with KD.
STUDY DESIGN
Stool specimens were obtained from 60 patients with KD and 62 age-matched children (36 children with acute illness and 26 healthy children). Total DNA was extracted from these stool samples. Using polymerase chain reaction, we examined genes of 5 SAgs: streptococcal pyrogenic exotoxin-A (SPE-A), SPE-C, SPE-G, SPE-J, and toxic shock syndrome toxin-1.
RESULTS
At least 1 of the 5 SAg genes was detected in 42 (70%) specimens from patients with KD, 14 (38.9%) from the febrile group, and 7 (26.9%) from the healthy group. The detection rate between subjects with and without KD was of at least 1 of the 5 SAg genes (P < .001), and more than 2 SAg genes were significantly different (P = .002).
CONCLUSIONS
SAg may be involved in the development of KD; data suggest that multiple SAgs may trigger KD.
目的
通过检测川崎病（KD）患者粪便中的超抗原（SAg）基因，研究SAg是否参与KD的发病过程。
研究设计
收集60例KD患者以及62例年龄匹配儿童（36例急性病患儿和26例健康儿童）的粪便标本。从这些粪便样本中提取总DNA。采用聚合酶链反应，检测5种SAg的基因：链球菌致热外毒素A（SPE-A）、SPE-C、SPE-G、SPE-J和中毒性休克综合征毒素-1。
结果
KD患者的42份（70%）标本、发热组的14份（38.9%）标本以及健康组的7份（26.9%）标本中检测到至少1种5种SAg基因。KD患者与非KD患者之间5种SAg基因中至少1种的检测率（P <.001），以及超过2种SAg基因的检测率有显著差异（P =.002）。
结论
SAg可能参与KD的发病过程；数据表明多种SAg可能引发KD。
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