Wen Hai-yan, Wang Jing, Liu Heng-chuan, Yang Yu, Hu Kong-xin, Sun Xiao-hong
China Academy of Inspection and Quarantin, Beijing 100025, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Mar;40(2):325-9.
To develop a rapid, high-throughput screening method of gene suspension array technique to simultaneously detect five bioterrorism bacteria: Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella spp. and Burkholderia pseudomallei.
Highly validated specific primers were used to amplify diagnostic regions unique to each pathogen. Biotin labelled PCR products were hybridized to corresponding probes coupling on the unique sets of fluorescent beads. The hybridized beads were processed through the Bio-plex, which identified the presence of PCR products.
Multiplex PCR-suspension array can detect five bioterrorism bacteria correctly with high specificity and high sensitivity, the results suggest the utility of suspension array system for high-throughput screening of bioterrorism samples.
A multiplex PCR-suspension array for rapid detection of five bioterrorism bacteria was established.
开发一种基因悬浮芯片技术的快速、高通量筛选方法，以同时检测五种生物恐怖主义相关细菌：炭疽芽孢杆菌、土拉弗朗西斯菌、鼠疫耶尔森菌、布鲁氏菌属和类鼻疽伯克霍尔德菌。
使用经过高度验证的特异性引物扩增每种病原体特有的诊断区域。生物素标记的PCR产物与偶联在独特荧光微珠组上的相应探针杂交。杂交后的微珠通过Bio-plex进行处理，以鉴定PCR产物的存在。
多重PCR-悬浮芯片能够以高特异性和高灵敏度正确检测五种生物恐怖主义相关细菌，结果表明悬浮芯片系统在生物恐怖主义样本高通量筛选中的实用性。
建立了一种用于快速检测五种生物恐怖主义相关细菌的多重PCR-悬浮芯片。
Zotero 插件