Roberti M Julia, Morgan Marcos, Menéndez Guillermo, Pietrasanta Lía I, Jovin Thomas M, Jares-Erijman Elizabeth A
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Buenos Aires, Argentina.
J Am Chem Soc. 2009 Jun 17;131(23):8102-7. doi: 10.1021/ja900225w.
Quantum dots multifunctionalized with the amyloid protein alpha-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only approximately 100 nanoparticles. The combination with a tetracysteine-tagged form of alpha-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells.
用淀粉样蛋白α-突触核蛋白多功能化的量子点在纳摩尔浓度下，可在体外和细胞中作为微摩尔至毫摩尔浓度的该蛋白聚集的非常有效的诱导剂。活细胞中的纤维化是帕金森病的诊断过程，仅约100个纳米颗粒就能将其加速多达15倍。与对荧光双砷化合物具有特异性的四半胱氨酸标记形式的α-突触核蛋白相结合，构成了一个研究细胞中病理性淀粉样蛋白形成的非常敏感的系统。
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