Kotani N, Tsugita A, Kondo K, Aso K, Enami I
Research Institute for Biosciences, Faculty of Science and Technology, Science University of Tokyo, Noda, Japan.
Protein Seq Data Anal. 1991 Aug;4(2):93-6.
Four small subunits (14, 13, 10, and 8 kDa) of the photosystem I reaction-center complex were isolated from a thermophilic cyanobacterium Synechococcus elongatus. The complete amino acid sequence of the 10-kDa subunit was determined to consist of 80 amino acid residues giving a molecular mass of 8855.3, excluding iron and sulfur atoms, and containing two special sequences of cysteine residues, Cys-X-X-Cys-X-X-Cys-X-X-X-Cys-Pro, at residues 10-21 and 47-58, which indicates that the subunit is an apoprotein carrying two iron-sulfur centers, FA and FB, assigned as [4Fe-4S] clusters. The amino acid sequence indicated an 87.5% identity compared with those deduced from the nucleotide sequences of chloroplast gene psa C from several plants.
从嗜热蓝藻聚球藻中分离出了光系统I反应中心复合物的四个小亚基（14、13、10和8 kDa）。确定10 kDa亚基的完整氨基酸序列由80个氨基酸残基组成，分子量为8855.3（不包括铁和硫原子），并在第10 - 21位和47 - 58位残基处含有两个半胱氨酸残基的特殊序列Cys-X-X-Cys-X-X-Cys-X-X-X-Cys-Pro，这表明该亚基是一种携带两个铁硫中心FA和FB的脱辅基蛋白，被指定为[4Fe-4S]簇。该氨基酸序列与几种植物叶绿体基因psa C的核苷酸序列推导的序列相比，同一性为87.5%。
