在小鼠腺病毒的辅助下，腺相关病毒对小鼠成纤维细胞的感染
Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus.
作者信息
Bhrigu Vipul, Trempe James P
机构信息
Department of Biochemistry and Cancer Biology, University of Toledo, College of Medicine, USA.
出版信息
Virology. 2009 Jul 20;390(1):22-30. doi: 10.1016/j.virol.2009.04.020. Epub 2009 May 21.
Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant particles than Hela cells. Levels of AAV-2 DNA replication were approximately 30-fold less in 3T3 cells as compared to Hela cells coinfected with human adenovirus (Ad-5). A study of these lower levels of infection in 3T3 cells compared to Hela cells revealed that receptor binding and internalization of AAV-2 in 3T3 and Hela cells was comparable. However, AAV-2 did not enter into the nucleus of mouse cells as efficiently as it does in human cells. Furthermore, viral DNA replication levels of AAV-2 DNA were found to be lower in mouse cells than human cells, indicating limitations in the murine nucleus for viral replication.
当宿主细胞与辅助病毒共感染时，腺相关病毒（AAV-2）可复制至高滴度。在此，我们分析了AAV-2与小鼠腺病毒（MAV-1）在鼠成纤维细胞中的共感染情况。我们观察到，AAV-2/MAV-1共感染的NIH 3T3细胞产生的AAV-2抗DNA酶颗粒比Hela细胞少约10 - 40倍。与感染人腺病毒（Ad-5）的Hela细胞相比，3T3细胞中AAV-2 DNA复制水平约低30倍。对3T3细胞与Hela细胞中这种较低感染水平的研究表明，AAV-2在3T3细胞和Hela细胞中的受体结合及内化情况相当。然而，AAV-2进入小鼠细胞核的效率不如进入人细胞核的效率高。此外，发现AAV-2 DNA在小鼠细胞中的病毒DNA复制水平低于人细胞，这表明小鼠细胞核中存在病毒复制的限制。
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