Girardeau Sarah, Mine Solène, Pageon Hervé, Asselineau Daniel
L'Oréal Research, 92583 Clichy cedex, France.
Exp Dermatol. 2009 Aug;18(8):704-11. doi: 10.1111/j.1600-0625.2009.00843.x. Epub 2009 Mar 12.
In the literature, most reported differences between African and Caucasian skin properties concern pigmentation and barrier function of the stratum corneum and related photoprotective properties. However, little is known about differences in morphology and possibly related biological functions. In this study, we investigated: (i) architectural differences of Caucasian and African mammary skin biopsies using microscopy, (ii) comparative constitutive expression of cytokines, matrix metalloproteinase 1 (MMP-1) and its inhibitors in papillary dermal fibroblast (pF) and reticular dermal fibroblast (rF) cultures in order to reveal biological features. (i) Neither epidermis thickness nor superficial dermis thickness was significantly different in African versus Caucasian subjects. However, the dermal-epidermal junction (DEJ) length in African skin was about threefold that in Caucasian skin. No differences were noticed as regards elastic and collagen fibre organization. (ii) In papillary fibroblast cultures, a significantly higher level of monocyte chemotactic peptide-1 (MCP-1) protein was found in cell cultures from African donors when compared with that from Caucasians. With regard to keratinocyte growth factor (KGF), the ratio of papillary to reticular fibroblast expression was found to be twofold greater in cell cultures from African donors compared with that from Caucasian donors. The same trend was found regarding MMP-1 and tissue inhibitor metalloproteinase protein 1 (TIMP-1) protein expression. African skin displays a greater convolution of the DEJ and a higher papillary fibroblast activity. These findings reveal that differences between African and Caucasian skin do not only affect upper epidermis but also dermal functions and dermal-epidermal cellular interactions.
在文献中，大多数报道的非洲人与白种人皮肤特性的差异涉及角质层的色素沉着、屏障功能及相关的光保护特性。然而，对于形态学差异以及可能相关的生物学功能却知之甚少。在本研究中，我们调查了：（i）使用显微镜观察白种人和非洲人乳腺皮肤活检组织的结构差异；（ii）比较乳头层真皮成纤维细胞（pF）和网状层真皮成纤维细胞（rF）培养物中细胞因子、基质金属蛋白酶1（MMP-1）及其抑制剂的组成性表达，以揭示生物学特征。（i）非洲人与白种人受试者的表皮厚度和浅表真皮厚度均无显著差异。然而，非洲人皮肤的真皮-表皮连接（DEJ）长度约为白种人皮肤的三倍。在弹性纤维和胶原纤维组织方面未发现差异。（ii）在乳头层成纤维细胞培养物中，与白种人相比，非洲供体的细胞培养物中单核细胞趋化肽-1（MCP-1）蛋白水平显著更高。关于角质形成细胞生长因子（KGF），发现非洲供体细胞培养物中乳头层与网状层成纤维细胞的表达比例是白种人供体细胞培养物的两倍。在MMP-1和金属蛋白酶组织抑制剂蛋白1（TIMP-1）蛋白表达方面也发现了相同趋势。非洲人皮肤的DEJ卷曲度更大，乳头层成纤维细胞活性更高。这些发现表明，非洲人和白种人皮肤之间的差异不仅影响表皮上层，还影响真皮功能以及真皮-表皮细胞相互作用。
