Shiseido Laboratories, 1050 Nippa-Cho, Kohoku-Ku, Yokohama-Shi, Japan 223.
Int J Cosmet Sci. 1984 Feb;6(1):33-46. doi: 10.1111/j.1467-2494.1984.tb00356.x.
Synopsis Protein denaturation was investigated to establish an in vitro evaluation method of surfactants in connection with their in vivo irritation potency to human skin. Eventually a new method with simplicity and high reproducibility was established by using quantitative analysis with gel-permeation chromatography (GPC). The protein denaturing potency of the commercially available surfactants was measured by using the developed method. Synergistic reduction in protein denaturation was observed in the mixed systems of anionic and amphoteric surfactants. The synergistic reduction was explained in terms of the physico-chemical properties of the mixed surfactants. A possible mechanism is the remarkable lowering of the total monomer concentration by the formation of hydrophobic complexes between the anionic and amphoteric surfactants.
摘要 为了建立一种与体内对人体皮肤刺激性相关的表面活性剂的体外评价方法，研究了蛋白质变性。最终，通过使用凝胶渗透色谱（GPC）进行定量分析，建立了一种简单、重现性高的新方法。利用所开发的方法测量了市售表面活性剂的蛋白质变性能力。在阴离子和两性表面活性剂的混合体系中观察到蛋白质变性的协同降低。协同降低可以用混合表面活性剂的物理化学性质来解释。一种可能的机制是阴离子和两性表面活性剂之间形成疏水性配合物，显著降低了总单体浓度。
