Clermont Olivier, Dhanji Hiran, Upton Mathew, Gibreel Tarek, Fox Andrew, Boyd David, Mulvey Michael R, Nordmann Patrice, Ruppé Etienne, Sarthou Jean Louis, Frank Thierry, Vimont Sophie, Arlet Guillaume, Branger Catherine, Woodford Neil, Denamur Erick
INSERM U722 and Université Paris 7, Faculté de Médecine, Site Xavier Bichat, 75018 Paris, France.
J Antimicrob Chemother. 2009 Aug;64(2):274-7. doi: 10.1093/jac/dkp194. Epub 2009 May 27.
Recently, a CTX-M-15 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone.
A total of 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene.
One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris.
We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.
最近，一种产CTX-M-15超广谱β-内酰胺酶（ESBL）的大肠埃希菌O25b-ST131克隆株在全球范围内被报道，该克隆株属于B2系统发育群，具有高毒力潜力，是一个重大的公共卫生问题。本研究旨在开发一种快速简便的检测方法来鉴定该克隆株的成员。
使用针对pabB基因的O25b-ST131克隆等位基因特异性PCR，对来自四大洲的总共627株大肠埃希菌分离株进行筛选，其中373株产ESBL。
143株产ESBL的分离株检测呈阳性。这些分离株均为O25b型，经多位点序列分型研究（25例），均为ST131型。发现O25b-ST131克隆株除产生CTX-M-15外，还产生其他ESBL，特别是CTX-M-2、-3、-14、-27、-32和-61以及TEM-24。该克隆株占巴黎尿路感染来源的非ESBL B2分离株的3%。
我们开发了一种基于PCR的检测方法，可轻松鉴定出极有可能产ESBL（包括CTX-M-15）的克隆株。
