Chouikha A, Fodha I, Ben Hadj Fredj M, Ardhaoui M, Teleb N, Brini I, Messaadi F, Mastouri M, Sfar T, Hachicha M, Kammoun T, Bouaaziz A, Amri F, Harbi A, Zribi M, Bousnina S, Khemakhem A, Boujaafar N, Trabelsi A, Steele A D
Laboratory of Transmitted Diseases (MDT-01), Faculty of Pharmacy, Monastir, Tunisia.
Pathol Biol (Paris). 2011 Jun;59(3):e43-8. doi: 10.1016/j.patbio.2009.04.008. Epub 2009 May 29.
Rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to detect a relationship between electropherotype pattern and molecular characteristics of the rotavirus strains.
Were analyzed 278 rotavirus-positive specimens by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR. Pearson's correlation tests were used for statistical analysis.
Twelve different electropherotypes were visualized, eight with a long profile (186 cases) and four with a short one (87 cases). Concerning VP7 types, G2 viral strains were found to be predominant and were detected in 91 specimens (32.7%). Strains with G1, G3, G4, G8 and G9 specificities were detected in 62 (22.3%), 82 (29.5%), 13 (4.7%), two (0.7%) and seven cases (2.5%), respectively. The results of VP4 genotyping showed a predominance of P[8] genotype which comprised half of the strains identified (139 cases, 50%). VP4 P[4], P[6] and P[11] were found in 83 (29.9%), 31 (11.1%) and 11 (4.0%) specimens, respectively. A high rate of mixed strains was also found (1.8% mixed electropherotypes, 7.6% G-mixed and 5% P-mixed strains). Electropherotype pattern of rotavirus strains was significantly correlated with VP7 genotype (p=0.018) and with VP4 genotype specificities (p<0.001).
轮状病毒是全球儿童腹泻最常见的病原体。通过聚丙烯酰胺凝胶电泳（PAGE）分析基因组RNA的11个片段的迁移率，可产生特定轮状病毒分离株特有的图谱。A组轮状病毒可通过分别分析负责将轮状病毒分类为G和P基因型的VP7和VP4基因特异性进一步进行特征鉴定。本研究的目的是检测轮状病毒株的电泳图谱模式与分子特征之间的关系。
通过PAGE分析278份轮状病毒阳性标本，并通过多重半巢式RT-PCR进行G/P基因分型。采用Pearson相关检验进行统计分析。
观察到12种不同的电泳图谱模式，8种为长图谱（186例），4种为短图谱（87例）。关于VP7类型，发现G2病毒株占主导地位，在91份标本（32.7%）中检测到。分别在62例（22.3%）、82例（29.5%）、13例（4.7%）、2例（0.7%）和7例（2.5%）中检测到具有G1、G3、G4、G8和G9特异性的毒株。VP4基因分型结果显示P[8]基因型占主导地位，占鉴定毒株的一半（139例，50%）。分别在83份（29.9%）标本、31份（11.1%）标本和11份（4.0%）标本中发现VP4 P[4]、P[6]和P[11]。还发现了较高比例的混合毒株（1.8%混合电泳图谱模式、7.6% G混合和5% P混合毒株）。轮状病毒株的电泳图谱模式与VP7基因型（p = 0.018）和VP4基因型特异性（p < 0.001）显著相关。
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