Hopwood J J, Dorfman A
J Biol Chem. 1977 Jul 25;252(14):4777-85.
The synthesis of glycosaminoglycans by human skin fibroblasts derived from normal subjects, Hurler and Marfan patients before and after transformation by SV40 virus has been studied. Virus transformation results in a marked increase in hyaluronic acid synthesis in normal and Hurler fibroblasts and, to a lesser extent, in Marfan fibroblasts which show augmented synthesis of this polysaccharide before transformation. There is also an increase in heparan sulfate synthesis but a moderate decrease in dermatan sulfate synthesis on transformation. Incubation of transformed fibroblasts with 4-methylumbelliferyl-beta-D-xyloside results in a marked increase in synthesis of free chondroitin sulfate chains. The synthesis of hyaluronic acid, but not of dermatan sulfate, is inversely proportional to cell density in normal fibroblasts but not in transformed fibroblasts.
对来自正常受试者、胡勒氏综合征患者和马凡氏综合征患者的人皮肤成纤维细胞在被SV40病毒转化前后的糖胺聚糖合成情况进行了研究。病毒转化导致正常和胡勒氏综合征成纤维细胞中透明质酸合成显著增加，在马凡氏综合征成纤维细胞中增加程度较小，而马凡氏综合征成纤维细胞在转化前该多糖的合成就已增强。转化后硫酸乙酰肝素合成也增加，但硫酸皮肤素合成适度减少。用4-甲基伞形酮基-β-D-木糖苷孵育转化的成纤维细胞会导致游离硫酸软骨素链的合成显著增加。在正常成纤维细胞中，透明质酸而非硫酸皮肤素的合成与细胞密度成反比，但在转化的成纤维细胞中并非如此。
