Chang Xiao-hong, Zhang Li, Yang Rong, Feng Jie, Cheng Ye-xia, Cheng Hong-yan, Ye Xue, Fu Tian-yun, Cui Heng
Gynecologic Oncology Center, Peking University People's Hospital, Beijing, China.
Chin Med J (Engl). 2009 May 20;122(10):1167-72.
Human epithelial ovarian cancer cell line SKOV3.ip1 is more invasive and metastatic compared with its parental line SKOV3. A total of 17 000 human genome complementary DNA microarrays were used to compare the gene expression patterns of the two cell lines. Based on this, the gene expression profiles of 22 patients with ovarian cancer were analyzed by cDNA microarray, and screened the 2-fold differentially expressed genes compared with the normal ones. We screened genes relevant to clinical prognosis of serous ovarian cancer by determining the expression profiles of ovarian cancer genes to investigate cell receptor and immunity-associated genes, and as groundwork, identify ovarian cancer-associated antigens at the gene level.
Total RNA was extracted from 22 patients with ovarian cancer and DNA microarrays were prepared. After scanning, hybridization signals were collected and the genes that were differentially expressed twice as compared with the normal ones were screened.
We screened 236 genes relevant to the prognosis of ovarian cancer from the 17 000 human genome cDNA microarrays. According to gene classification, 48 of the 236 genes were cell receptor or immunity-associated genes, including 2 genes related to the International Federation of Gynecology and Obstetrics (FIGO) stage, 4 genes to histological grade, 18 genes to lymph node metastasis, 11 genes to residual disease, and 13 genes to the reactivity to chemotherapy. Several functionally important genes including fibronectin 1, pericentriolar material 1, beta-2-microglobulin, PPAR binding protein were identified through review of the literature.
The cDNA microarray of ovarian cancer genes developed in this study was effective and high throughput in screening the ovarian cancer-associated genes differentially expressed. Through the studies of the cell receptor and immunity-associated genes we expect to identify the molecular biology index of ovarian cancer-associated antigens.
人上皮性卵巢癌细胞系SKOV3.ip1与其亲代细胞系SKOV3相比，具有更强的侵袭性和转移性。使用总共17000个人类基因组互补DNA微阵列来比较这两种细胞系的基因表达模式。在此基础上，通过cDNA微阵列分析22例卵巢癌患者的基因表达谱，并筛选出与正常组织相比差异表达2倍的基因。通过确定卵巢癌基因的表达谱来筛选与浆液性卵巢癌临床预后相关的基因，以研究细胞受体和免疫相关基因，并作为基础在基因水平上鉴定卵巢癌相关抗原。
从22例卵巢癌患者中提取总RNA并制备DNA微阵列。扫描后，收集杂交信号并筛选出与正常组织相比差异表达两倍的基因。
我们从17000个人类基因组cDNA微阵列中筛选出236个与卵巢癌预后相关的基因。根据基因分类，236个基因中的48个是细胞受体或免疫相关基因，包括2个与国际妇产科联盟（FIGO）分期相关的基因、4个与组织学分级相关的基因、18个与淋巴结转移相关的基因、11个与残留病灶相关的基因以及13个与化疗反应相关的基因。通过文献回顾鉴定出了几个功能重要的基因，包括纤连蛋白1、中心粒周物质1、β2微球蛋白、PPAR结合蛋白。
本研究开发的卵巢癌基因cDNA微阵列在筛选差异表达的卵巢癌相关基因方面有效且高通量。通过对细胞受体和免疫相关基因的研究，我们期望鉴定出卵巢癌相关抗原的分子生物学指标。
