Hacker Kari, White Laura, de Silva Aravinda M
Department of Microbiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
J Gen Virol. 2009 Sep;90(Pt 9):2097-106. doi: 10.1099/vir.0.012120-0. Epub 2009 Jun 3.
This study compared the ability of mosquito and mammalian cell-derived dengue virus (DENV) to infect human dendritic cell-specific ICAM3-grabbing non-integrin (DC-SIGN)-expressing cells and characterized the structure of envelope (E) protein N-linked glycans on DENV derived from the two cell types. DENVs derived from both cell types were equally effective at infecting DC-SIGN-expressing human monocytes and dendritic cells. The N-linked glycans on mosquito cell-derived virus were a mix of high-mannose and paucimannose glycans. In virus derived from mammalian cells, the N-linked glycans were a mix of high-mannose and complex glycans. These results indicate that N-linked glycans are incompletely processed during DENV egress from cells, resulting in high-mannose glycans on viruses derived from both cell types. Studies with full-length and truncated E protein demonstrated that incomplete processing was most likely a result of the poor accessibility of glycans on the membrane-anchored protein.
本研究比较了蚊子细胞和哺乳动物细胞衍生的登革病毒（DENV）感染表达人树突状细胞特异性细胞间黏附分子3结合非整合素（DC-SIGN）的细胞的能力，并对源自这两种细胞类型的DENV包膜（E）蛋白N-连接聚糖的结构进行了表征。源自这两种细胞类型的DENV在感染表达DC-SIGN的人单核细胞和树突状细胞方面同样有效。蚊子细胞衍生病毒上的N-连接聚糖是高甘露糖聚糖和寡甘露糖聚糖的混合物。在源自哺乳动物细胞的病毒中，N-连接聚糖是高甘露糖聚糖和复合聚糖的混合物。这些结果表明，N-连接聚糖在DENV从细胞中释放过程中加工不完全，导致源自这两种细胞类型的病毒上都有高甘露糖聚糖。对全长和截短E蛋白的研究表明，加工不完全很可能是由于膜锚定蛋白上聚糖的可及性较差所致。
