羟基自由基参与细菌拓扑异构酶I切割复合物的细胞杀伤作用。
Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex.
作者信息
Liu I-Fen, Annamalai Thirunavukkarasu, Sutherland Jeanette H, Tse-Dinh Yuk-Ching
机构信息
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.
出版信息
J Bacteriol. 2009 Aug;191(16):5315-9. doi: 10.1128/JB.00559-09. Epub 2009 Jun 12.
Abstract
Escherichia coli expressing SOS-inducing mutant topoisomerase I was utilized to demonstrate that covalent protein-DNA complex accumulation results in oxidative damage. Hydroxyl radicals were detected following mutant topoisomerase induction. The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing.
摘要
表达诱导SOS的突变拓扑异构酶I的大肠杆菌被用于证明共价蛋白-DNA复合物的积累会导致氧化损伤。在诱导突变拓扑异构酶后检测到了羟基自由基。Fe(2+)螯合剂2,2'-联吡啶的存在以及影响铁硫簇形成的iscS突变可保护细胞免受拓扑异构酶I切割复合物介导的细胞杀伤。
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2
Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli.
J Mol Biol. 2009 Jan 16;385(2):558-67. doi: 10.1016/j.jmb.2008.10.073. Epub 2008 Nov 5.
3
DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.
Q Rev Biophys. 2008 Feb;41(1):41-101. doi: 10.1017/S003358350800468X.
4
Inhibition of Mg2+ binding and DNA religation by bacterial topoisomerase I via introduction of an additional positive charge into the active site region.
Nucleic Acids Res. 2008 Aug;36(14):4788-96. doi: 10.1093/nar/gkn460. Epub 2008 Jul 24.
5
SOS induction by stabilized topoisomerase IA cleavage complex occurs via the RecBCD pathway.
J Bacteriol. 2008 May;190(9):3399-403. doi: 10.1128/JB.01674-07. Epub 2008 Feb 29.
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Mutation adjacent to the active site tyrosine can enhance DNA cleavage and cell killing by the TOPRIM Gly to Ser mutant of bacterial topoisomerase I.
Nucleic Acids Res. 2008 Feb;36(3):1017-25. doi: 10.1093/nar/gkm1126. Epub 2007 Dec 20.
7
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9
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10
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