蜂胶对大鼠雨蛙肽诱导的实验性急性胰腺炎的有益作用。

The beneficial effect of propolis on cerulein-induced experimental acute pancreatitis in rats.

作者信息

Büyükberber Mehmet, Savaş M Cemil, Bağci Cahit, Koruk Mehmet, Gülşen M Taner, Tutar Ediz, Bilgiç Tuğba, Deveci Rukiye, Küçük Can

机构信息

Department of Gastroenterology, Gaziantep University, School of Medicine, Gaziantep, Turkey.

出版信息

Turk J Gastroenterol. 2009 Jun;20(2):122-8.

PMID:19530045

Abstract

BACKGROUND/AIMS: Inflammatory cytokines and oxidative stress have a central role in the pathogenesis of acute pancreatitis. Propolis is a resinous hive product collected by honeybees from various plant sources and has anti-inflammatory and anti-oxidant effects. The present work aimed to investigate the therapeutic role of ethanolic extract of propolis on a cerulein-induced acute pancreatitis model in rats.

METHODS

Seventy male Wistar albino rats were used in the study. Acute edematous pancreatitis was induced by subcutaneous cerulein injection (20 microg/kg) four times at one-hour intervals. Ethanolic extract of propolis 300 mg/kg was given subcutaneously at the beginning of the procedure (ethanolic extract of propolis-1 group) or 12 h after the last cerulein injection (ethanolic extract of propolis-2 group). Serum amylase and lipase levels, white blood cell count and serum tumor necrosis factor-alpha levels were measured and pancreatic tissue was evaluated histologically.

RESULTS

In the acute pancreatitis group, serum amylase and lipase levels were found to be elevated and the histopathological evaluation of the tissue revealed massive edema and inflammation with less fatty necrosis when compared to the sham and control groups. Serum amylase and lipase levels and edema formation were significantly decreased in the ethanolic extract of propolis-treated groups (p<0.001). In the ethanolic extract of propolis-2 group, in particular, tissue edema was improved markedly (p=0.001). Tissue inflammation and fatty necrosis were decreased with ethanolic extract of propolis treatment; however, the improvement was not statistically significant.

CONCLUSIONS

Treatment with ethanolic extract of propolis improved the biochemical and histopathological findings in a rat model of experimental pancreatitis. Although our findings suggest that ethanolic extract of propolis might be considered an effective agent for the treatment of acute pancreatitis, this notion should be supported with further experimental and clinical investigations.

摘要

背景/目的：炎性细胞因子和氧化应激在急性胰腺炎的发病机制中起核心作用。蜂胶是蜜蜂从多种植物来源采集的树脂状蜂巢产物，具有抗炎和抗氧化作用。本研究旨在探讨蜂胶乙醇提取物对大鼠雨蛙肽诱导的急性胰腺炎模型的治疗作用。

方法

本研究使用了70只雄性Wistar白化大鼠。通过皮下注射雨蛙肽（20微克/千克），每隔1小时注射1次，共注射4次，诱导急性水肿性胰腺炎。在实验开始时（蜂胶乙醇提取物-1组）或最后一次注射雨蛙肽后12小时（蜂胶乙醇提取物-2组），皮下给予300毫克/千克的蜂胶乙醇提取物。测量血清淀粉酶和脂肪酶水平、白细胞计数以及血清肿瘤坏死因子-α水平，并对胰腺组织进行组织学评估。

结果

在急性胰腺炎组中，发现血清淀粉酶和脂肪酶水平升高，与假手术组和对照组相比，组织的组织病理学评估显示大量水肿和炎症，脂肪坏死较少。蜂胶乙醇提取物治疗组的血清淀粉酶和脂肪酶水平以及水肿形成明显降低（p<0.001）。特别是在蜂胶乙醇提取物-2组中，组织水肿明显改善（p=0.001）。蜂胶乙醇提取物治疗可减轻组织炎症和脂肪坏死；然而，改善情况无统计学意义。