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铜绿假单胞菌CCTCC AB91095纯培养物中六价铬的同步还原与苯酚降解

Simultaneous Cr(VI) reduction and phenol degradation in pure cultures of Pseudomonas aeruginosa CCTCC AB91095.

作者信息

Song Huaxiao, Liu Yunguo, Xu Weihua, Zeng Guangming, Aibibu Nuzaaiti, Xu Li, Chen Beibei

机构信息

College of Environmental Science and Engineering, Hunan University, Lushan Road, Changsha 410082, China.

出版信息

Bioresour Technol. 2009 Nov;100(21):5079-84. doi: 10.1016/j.biortech.2009.05.060. Epub 2009 Jun 21.

Abstract

Simultaneous Cr(VI) reduction and phenol degradation were investigated in a reactor containing Pseudomonas aeruginosa CCTCC AB91095. Phenol was used as carbon source. P.aeruginosa utilized metabolites formed during phenol degradation as energy source for Cr(VI) reduction. Cr(VI) inhibited both Cr(VI) reduction and phenol degradation when Cr(VI) concentration exceeded the optimum value (20 mg/L), whereas phenol enhanced both Cr(VI) reduction and phenol degradation below the optimum initial concentration of 100 mg/L. Cr(III) was the predominant product of Cr(VI) reduction in cultures after incubation for 24 h. Both Cr(VI) reduction and phenol degradation were influenced by the amount of inocula. The concentration of Cr(VI) and phenol declined quickly from 20, 100 to 3.36, 29.51 mg/L in cultures containing of 5% (v/v) inoculum after incubation for 12 h, respectively. The whole study showed that P. aeruginosa is promising for the reduction of toxic Cr(VI) and degradation of organic pollutants simultaneously in the mineral liquid medium.

摘要

在含有铜绿假单胞菌CCTCC AB91095的反应器中研究了六价铬(Cr(VI))的同步还原和苯酚降解。苯酚用作碳源。铜绿假单胞菌利用苯酚降解过程中形成的代谢产物作为六价铬还原的能源。当六价铬浓度超过最佳值(20 mg/L)时,六价铬会抑制六价铬还原和苯酚降解,而在初始浓度低于100 mg/L的最佳值时,苯酚会促进六价铬还原和苯酚降解。培养24小时后,三价铬(Cr(III))是培养物中六价铬还原的主要产物。六价铬还原和苯酚降解均受接种量的影响。在含有5%(v/v)接种物的培养物中培养12小时后,六价铬和苯酚的浓度分别从20、100迅速降至3.36、29.51 mg/L。整个研究表明,在矿物液体培养基中,铜绿假单胞菌在同时还原有毒的六价铬和降解有机污染物方面具有前景。

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