MDMA（摇头丸）会减少胚胎皮质培养物中的神经元和干细胞数量。
MDMA (Ecstasy) decreases the number of neurons and stem cells in embryonic cortical cultures.
机构信息
Department of Neuroscience, Uppsala University, Uppsala, Sweden.
出版信息
Cell Mol Neurobiol. 2010 Jan;30(1):13-21. doi: 10.1007/s10571-009-9426-y. Epub 2009 Jun 19.
Ecstasy, 3,4-methylenedioxymetamphetamine (MDMA), is a recreational drug used among adolescents, including young pregnant women. MDMA passes the placental barrier and may therefore influence fetal development. The aim was to investigate the direct effect of MDMA on cortical cells using dissociated CNS cortex of rat embryos, E17. The primary culture was exposed to a single dose of MDMA and collected 5 days later. MDMA caused a dramatic, dose-dependent (100 and 400 microM) decrease in nestin-positive stem cell density, as well as a significant reduction (400 microM) in NeuN-positive cells. By qPCR, MDMA (200 microM) caused a significant decrease in mRNA expression of the 5HT3 receptor, dopamine D(1) receptor, and glutamate transporter EAAT2-1, as well as an increase in mRNA levels of the NMDA NR1 receptor subunit and the 5HT(1A) receptor. In conclusion, MDMA caused a marked reduction in stem cells and neurons in embryonic cortical primary cell cultures, which was accompanied by changes in mRNA expression of specific receptors and transporters for glutamatergic and monoaminergic neurotransmitters.
摇头丸，即 3,4-亚甲二氧基甲基苯丙胺（MDMA），是一种在青少年中，包括年轻孕妇中使用的消遣性药物。MDMA 可以穿过胎盘屏障，因此可能会影响胎儿发育。本研究的目的是使用分离的大鼠胚胎 E17 中枢神经系统皮质细胞，来检测 MDMA 对皮质细胞的直接影响。原代培养细胞接受 MDMA 单次处理，并在 5 天后收集细胞。结果显示 MDMA 导致巢蛋白阳性的干细胞密度显著、剂量依赖性下降（100 和 400μM），并显著减少神经元特异性核蛋白阳性细胞（400μM）。通过 qPCR 检测，200μM MDMA 导致 5HT3 受体、多巴胺 D1 受体和谷氨酸转运体 EAAT2-1 的 mRNA 表达显著下降，同时 NMDA NR1 受体亚单位和 5HT1A 受体的 mRNA 水平升高。综上所述，MDMA 导致胚胎皮质原代细胞培养中干细胞和神经元的显著减少，同时伴随谷氨酸能和单胺能神经递质特定受体和转运体的 mRNA 表达变化。
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