使用生物芯片系统快速、准确地测定结核分枝杆菌分离株和痰液中的多重耐药性。

Rapid, accurate determination of multidrug resistance in M. tuberculosis isolates and sputum using a biochip system.

作者信息

Guo Y, Zhou Y, Wang C, Zhu L, Wang S, Li Q, Jiang G, Zhao B, Huang H, Yu H, Xing W, Mitchelson K, Cheng Jing, Zhao Yanlin

机构信息

Medical Systems Biology Research Centre, Tsinghua University, Beijing, China.

出版信息

Int J Tuberc Lung Dis. 2009 Jul;13(7):914-20.

PMID:19555544

Abstract

OBJECTIVE

To develop and evaluate a rapid biochip system for the determination of multidrug-resistant tuberculosis (MDR-TB) in Mycobacterium tuberculosis isolates and clinical sputum samples.

DESIGN

We developed a total solution-based system, including a biochip kit, apparatus for sample preparation, hybridisation, washing and data acquisition, and dedicated software for automated diagnosis. The biochip simultaneously identifies M. tuberculosis and detects the most commonly found mutations in the rpoB, katG and inhA genes. The system was assessed with 330 mycobacterial isolates and 129 sputum samples for rifampicin (RMP), and with 205 isolates and 105 sputum samples for isoniazid (INH), and then compared to DNA sequencing and conventional drug susceptibility testing (DST).

RESULTS

The entire biochip assay took 6 h. The concordance rate between the biochip assay and the DNA sequencing results was 100%. Compared to conventional DST, the concordance rates were 91.8% for isolates and 94.6% for sputum samples for RMP resistance, and 70.2% for isolates and 78.1% for sputum samples for INH resistance.

CONCLUSION

The biochip system provides a simple, rapid, reliable and accurate clinical assay for the parallel detection of M. tuberculosis and prevalent MDR-TB in a 6 h procedure, using either culture isolates or sputum samples for diagnosis.

摘要

目的

开发并评估一种用于检测结核分枝杆菌分离株和临床痰液样本中耐多药结核病（MDR-TB）的快速生物芯片系统。

设计

我们开发了一种基于全解决方案的系统，包括生物芯片试剂盒、样本制备、杂交、洗涤和数据采集设备，以及用于自动诊断的专用软件。该生物芯片可同时鉴定结核分枝杆菌并检测rpoB、katG和inhA基因中最常见的突变。该系统用330株分枝杆菌分离株和129份痰液样本进行利福平（RMP）检测，用205株分离株和105份痰液样本进行异烟肼（INH）检测，然后与DNA测序和传统药敏试验（DST）进行比较。