[转化生长因子β3对实验性肝纤维化大鼠组织病理学及Ⅰ型胶原蛋白表达的影响]

[Effects of transforming growth factor beta 3 on the histopathology and expression of collagen I in experimental hepatic fibrotic rats].

作者信息

Liu Ping, Gao Xiao-liang, Yu Jiao, Qian Wei, Xu Ke-shu

机构信息

Department of Digestive Medicine, Union Hospital, Medical College, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2009 Jun;17(6):446-50.

PMID:19567025

Abstract

OBJECTIVE

To investgate the effects of TGFbeta3 on rat hepatic fibrosis.

METHODS

The TGFbeta3 cDNA was cloned into rAAV2 vector. Rats were randomly divided into four groups: normal control group, model group, negative control group and TGFbeta3 group. Hepatic fibrosis was induced by hypodermic injection of 40% CCl(4). Recombinant AAV2-TGFbeta3 viral particles were injected via the vena caudalis one week before CCl(4) treatment. Rats were executed 8 weeks after CCl(4) treatment, global histological change was observed after HE staining, the distribution of the collagen fibers was observed after masson staining, histochemistry was done to observe the expression of collagen I; The positive area rate of the collagen fibers and the average optical rate of collagen I were quantified.

RESULTS

HE staining indicated that collagen fibers were reduced in the TGFbeta3 group. Masson staining shown that the collagen fibers were distributed around the blood vessel, in the portal area and disse space. Compared to the model group (13.2%+/-2.2%) and negative control group (12.3%+/-1.5%), the collagen fibers in liver tissues of TGFbeta3 group (7.7%+/-1.5%) were significantly decreased (q = 9.456, P < 0.01; q = 8.217, P < 0.01). Histochemistry indicated that the collagen fibers of liver tissues of TGFbeta3 group (0.185+/-0.033) were significantly higher than those in the model group (0.252+/-0.042) and the negative control group (0.230+/-0.029), (q = 6.228, P < 0.01; q = 4.346, P < 0.01).

CONCLUSION

TGFbeta3 alleviates the damage to hepatic cell and the level of fibrosis in CCl(4) treated rats and inhibits the expression of collagen I.

摘要

目的

研究转化生长因子β3（TGFβ3）对大鼠肝纤维化的影响。

方法

将TGFβ3 cDNA克隆至重组腺相关病毒2型（rAAV2）载体。大鼠随机分为四组：正常对照组、模型组、阴性对照组和TGFβ3组。皮下注射40%四氯化碳（CCl4）诱导肝纤维化。在CCl4处理前一周，经尾静脉注射重组AAV2-TGFβ3病毒颗粒。CCl4处理8周后处死大鼠，苏木精-伊红（HE）染色观察整体组织学变化，马松（Masson）染色观察胶原纤维分布，进行组织化学观察Ⅰ型胶原表达；对胶原纤维阳性面积率和Ⅰ型胶原平均光密度率进行量化。

结果

HE染色显示TGFβ3组胶原纤维减少。Masson染色显示胶原纤维分布于血管周围、门管区和狄氏间隙。与模型组（13.2%±2.2%）和阴性对照组（12.3%±1.5%）相比，TGFβ3组肝组织胶原纤维（7.7%±1.5%）显著减少（q = 9.456，P < 0.01；q = 8.217，P < 0.01）。组织化学显示TGFβ3组肝组织胶原纤维（0.185±0.033）显著低于模型组（0.252±0.042）和阴性对照组（0.230±0.029）（q = 6.228，P < 0.01；q = 4.346，P < 0.01）。