Gao Jie, Ding Yi-ling
Department of Obstetrics and Gynecology, Xiangya Second Hospital, Central South University, Changsha 410011, China.
Zhonghua Fu Chan Ke Za Zhi. 2009 May;44(5):345-9.
To investigate the role of RhoA and Rho kinase system in the onset of labor.
Forty term pregnant women, who delivered through cesarean section at the Xiangya Second Hospital of Central South University from February 2007 to November 2007, were selected and divided into 2 groups: 20 in labor group and 20 in non-labor group. Another 20 non-pregnant women undergoing hysterectomy due to cervical intraepithelial neoplasia were chosen as the control. Real-time fluorescence quantitative RT-PCR and western blot were applied to detect the expression RhoA and ROCK I mRNA and protein in uterine smooth muscle tissue and the correlation between the mRNA and protein expression of RhoA and ROCK I were analyzed.
(1) The mRNA expressions of both RhoA and ROCK I were detected in all groups, and higher levels were found in the labor group than in the non-labor group and the control [RhoA mRNA: (3.51 +/- 0.56) x 10(-3) vs. (2.75 +/- 0.52) x 10(-3) and (2.11 +/- 0.54) x 10(-3); ROCK I mRNA: (4.07 +/- 0.66) x 10(-3) vs. (2.71 +/- 0.52) x 10(-3) and (2.01 +/- 0.23) x 10(-3), P < 0.01]. (2) RhoA and ROCK I proteins were also identified in all three groups, and the expressions in the labor and non-labor group were higher than those of the control (RhoA protein: 0.72 +/- 0.23 and 0.64 +/- 0.17 vs. 0.46 +/- 0.15; ROCK I protein: 0.56 +/- 0.14 and 0.42 +/- 0.16 vs. 0.29 +/- 0.08, P < 0.01). (3) The expression of RhoA mRNA and ROCK I mRNA were positively correlated in each of the three groups (r = 0.73, P < 0.01), and the same was found in the expression of RhoA protein and ROCKI protein (r = 0.37, P < 0.01).
The increased expression of RhoA and Rho kinase may play an important role in the initiation of labor.
探讨RhoA和Rho激酶系统在分娩发动中的作用。
选取2007年2月至2007年11月在中南大学湘雅二医院行剖宫产的40例足月孕妇，分为2组：临产组20例和未临产组20例。另选取因宫颈上皮内瘤变行子宫切除术的20例非孕妇作为对照组。采用实时荧光定量RT-PCR和蛋白质印迹法检测子宫平滑肌组织中RhoA和ROCK I mRNA及蛋白表达，并分析RhoA和ROCK I mRNA与蛋白表达的相关性。
(1) 所有组均检测到RhoA和ROCK I的mRNA表达，临产组表达水平高于未临产组和对照组 [RhoA mRNA：(3.51±0.56)×10⁻³ vs. (2.75±0.52)×10⁻³ 和 (2.11±0.54)×10⁻³；ROCK I mRNA：(4.07±0.66)×10⁻³ vs. (2.71±0.52)×10⁻³ 和 (2.01±0.23)×10⁻³，P<0.01]。(2) 三组均检测到RhoA和ROCK I蛋白，临产组和未临产组表达高于对照组 (RhoA蛋白：0.72±0.23和0.64±0.17 vs. 0.46±0.15；ROCK I蛋白：0.56±0.14和0.42±0.16 vs. 0.29±0.08，P<0.01)。(3) 三组中RhoA mRNA与ROCK I mRNA表达均呈正相关 (r = 0.73，P<0.01)，RhoA蛋白与ROCK I蛋白表达也呈正相关 (r = 0.37，P<0.01)。
RhoA和Rho激酶表达增加可能在分娩发动中起重要作用。
Zotero 插件