人源SOUL（一种血红素结合蛋白）的初步结构表征
Preliminary structural characterization of human SOUL, a haem-binding protein.
作者信息
Freire Filipe, Romão Maria João, Macedo Anjos L, Aveiro Susana S, Goodfellow Brian J, Carvalho Ana Luísa
机构信息
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt 7):723-6. doi: 10.1107/S174430910902291X. Epub 2009 Jun 27.
Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP(23) protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6(4)22, with unit-cell parameters a = b = 145, c = 60 A and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 A resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.
人SOUL（hSOUL）是一种23 kDa的血红素结合蛋白，最初被鉴定为从人足月胎盘中分离出的PP(23)蛋白。本文报道了hSOUL的过表达、纯化和结晶。晶体属于空间群P6(4)22，晶胞参数a = b = 145，c = 60 Å，不对称单位中有一个蛋白质分子。在欧洲同步辐射装置（ESRF）收集了分辨率为3.5 Å的X射线衍射数据。通过分子置换，以溶液核磁共振获得的小鼠p22HBP结构（PDB编号2gov和2hva）作为搜索模型，得到了hSOUL三维结构的初步模型。
Zotero 插件