高效液相色谱-直接电致化学发光检测法测定人尿中萘普生
Determination of naproxen in human urine by high-performance liquid chromatography with direct electrogenerated chemiluminescence detection.
作者信息
Sun Yonghua, Zhang Zhujun, Xi Zhijun, Shi Zuolong
机构信息
College of Chemistry and Materials Science, Shaanxi Normal University, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Xi'an 710062, China.
出版信息
Talanta. 2009 Aug 15;79(3):676-80. doi: 10.1016/j.talanta.2009.04.048. Epub 2009 May 3.
A simple and sensitive liquid chromatographic method coupled with electrogenerated chemiluminescence (ECL) was described for the separation and quantification of naproxen in human urine. The method was based on the ECL of naproxen in basic NaNO(3) solution with a dual-electrode system. Factors affected the ECL emission were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of naproxen in the range of 4.0 x 10(-8)g mL(-1) to 2.0 x 10(-6)g mL(-1) and the detection limit was 1.6 x 10(-8)g mL(-1) (S/N=3). Application of the method to the analyses of naproxen in human urine proved feasible.
描述了一种简单灵敏的液相色谱法结合电化学发光（ECL）用于分离和定量人尿中的萘普生。该方法基于萘普生在碱性NaNO₃溶液中使用双电极系统的ECL。研究了影响ECL发射的因素。在最佳条件下，ECL强度与萘普生浓度在4.0×10⁻⁸g mL⁻¹至2.0×10⁻⁶g mL⁻¹范围内呈线性关系，检测限为1.6×10⁻⁸g mL⁻¹（S/N = 3）。该方法应用于人尿中萘普生的分析证明是可行的。
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