The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Clin Biochem. 2009 Sep;42(13-14):1461-7. doi: 10.1016/j.clinbiochem.2009.06.021. Epub 2009 Jul 2.
A rapid and sensitive magnetic particles (MPs)-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for determination of luteinizing hormone (LH) in human serum was developed and validated.
The method was developed based on a sandwiched immunoreaction format, in which MPs served as both the solid phase and separator. The horseradish peroxidase(HRP)-luminol-H(2)O(2) chemiluminescent system was chosen as the detection system.
This method had a detection limit of 0.2 mIU/mL and a linear range of 0.5-200 mIU/mL. The assay showed little cross-reactivity, and the intra- and inter-assay coefficients of variation were less than 10%. The recoveries were from 101.6% to 104.1%. The method has been successfully applied to detection of LH in human serum and showed good correlation compared with radioimmunoassay (RIA).
The MPs-CLEIA provided apparent advantages over RIA, and facilitated the development of high-throughput screening and automated operation systems in clinical diagnosis.
建立一种基于磁微粒（MPs）的灵敏化学发光酶免疫分析（MPs-CLEIA）方法，用于测定人血清中的促黄体生成激素（LH）。
该方法基于夹心免疫反应模式，其中 MPs 既作为固相又作为分离相。选择辣根过氧化物酶（HRP）-鲁米诺-H（2）O（2）化学发光系统作为检测系统。
该方法的检测限为 0.2 mIU/mL，线性范围为 0.5-200 mIU/mL。该测定方法交叉反应性小，批内和批间变异系数均小于 10%。回收率为 101.6%-104.1%。该方法已成功应用于人血清中 LH 的检测，与放射免疫分析（RIA）相比具有良好的相关性。
与 RIA 相比，MPs-CLEIA 具有明显优势，有利于高通量筛选和临床诊断的自动化操作系统的发展。
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