通过铽配合物的色氨酸敏化发光对标签融合蛋白进行选择性标记。
Selective labeling of tag-fused protein by tryptophan-sensitized luminescence of a terbium complex.
作者信息
Hirayama Tasuku, Taki Masayasu, Kodan Atsushi, Kato Hiroaki, Yamamoto Yukio
机构信息
Graduate School of Human and Environmental Studies, Department of Structural Biology, Kyoto University, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.
出版信息
Chem Commun (Camb). 2009 Jun 14(22):3196-8. doi: 10.1039/b900302a. Epub 2009 May 6.
DOI:10.1039/b900302a
PMID:19587911
Abstract
A Tb3+ complex with two di(2-picolyl)amine (DPA) moieties and an oligo-aspartate peptide containing a tryptophan residue have been designed as a new binding pair for use in protein labeling.
摘要
一种含有两个二（2-吡啶甲基）胺（DPA）部分和一个含有色氨酸残基的寡聚天冬氨酸肽的Tb3+配合物已被设计为一种用于蛋白质标记的新型结合对。
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