Tai J H, Wang A L, Ong S J, Lai K S, Lo C, Wang C C
Molecular Parasitology Laboratory, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Exp Parasitol. 1991 Nov;73(4):413-23. doi: 10.1016/0014-4894(91)90065-5.
The subcellular distribution of Giardia lamblia virus RNA in infected G. lamblia trophozoites was examined by in situ hybridization using biotinylated DNA probe and riboprobe. In G. lamblia Portland I strain, which is chronically infected by G. lamblia viruses, the viral RNA was detected in the cytoplasm as well as in the twin nuclei. When riboprobe was used to examine the course of virus infection in WB strain, accumulation of viral RNA was detected only in the cytoplasm prior to the first 72 hr of infection. Using DNA probe, further accumulation of viral RNA in increasing number of cells occurred after the 72nd hr of infection, with the RNA found in both the cytoplasm and nuclei. Eventually, the cell nuclei showed damaged morphology that deteriorated rapidly toward the final stage of infection. These observations indicate that early phase of viral RNA replication may take place in the cytoplasm of infected G. lamblia, but the nuclei are also involved during the late phase of viral replication.
使用生物素化的DNA探针和核糖探针，通过原位杂交检测了蓝氏贾第鞭毛虫病毒RNA在感染的蓝氏贾第鞭毛虫滋养体中的亚细胞分布。在长期感染蓝氏贾第鞭毛虫病毒的波特兰I株蓝氏贾第鞭毛虫中，在细胞质以及双核中均检测到病毒RNA。当使用核糖探针检测WB株病毒感染过程时，在感染的前72小时内，仅在细胞质中检测到病毒RNA的积累。使用DNA探针，在感染72小时后，越来越多的细胞中病毒RNA进一步积累，RNA存在于细胞质和细胞核中。最终，细胞核显示出受损的形态，并在感染末期迅速恶化。这些观察结果表明，病毒RNA复制的早期阶段可能发生在受感染的蓝氏贾第鞭毛虫的细胞质中，但在病毒复制的后期细胞核也参与其中。
