Wnt蛋白在发育中的小鼠次生腭中的表达。
Expression of Wnts in the developing murine secondary palate.
作者信息
Warner Dennis R, Smith Henry S, Webb Cynthia L, Greene Robert M, Pisano M Michele
机构信息
University of Louisville Birth Defects Center, Department of Molecular, Cellular and Craniofacial Biology, Kentucky 40292, USA.
出版信息
Int J Dev Biol. 2009;53(7):1105-12. doi: 10.1387/ijdb.082578dw.
Abstract
Morphogenesis of the mammalian secondary palate requires coordination of cell migration, proliferation, differentiation, apoptosis and synthesis of extracellular matrix molecules by numerous signal transduction pathways. Recent evidence suggests a role for members of the Wnt family of secreted cytokines in orofacial development. However, no study has systematically or comprehensively examined the expression of Wnts in embryonic orofacial tissue. We thus conducted a survey of the expression of all known Wnt genes in the developing murine secondary palate. Using an RT-PCR strategy to assay gene expression, 12 of the 19 known members of the Wnt family were found to be expressed in embryonic palatal tissue during key phases of its development. The expression of 5 Wnt family members was found to be temporally regulated. Moreover, these Wnts had unique spatio-temporal patterns of expression which suggested possible roles in palatal ontogeny.
摘要
哺乳动物次生腭的形态发生需要众多信号转导途径协调细胞迁移、增殖、分化、凋亡以及细胞外基质分子的合成。最近的证据表明，分泌型细胞因子Wnt家族成员在口面部发育中发挥作用。然而，尚无研究系统全面地检测Wnts在胚胎口面部组织中的表达情况。因此，我们对发育中的小鼠次生腭中所有已知Wnt基因的表达进行了调查。采用逆转录聚合酶链反应（RT-PCR）策略检测基因表达，发现Wnt家族19个已知成员中的12个在胚胎腭组织发育的关键阶段有表达。发现5个Wnt家族成员的表达受时间调控。此外，这些Wnts具有独特的时空表达模式，提示其在腭个体发生中可能发挥的作用。
相似文献
1
Expression of Wnts in the developing murine secondary palate.Wnt蛋白在发育中的小鼠次生腭中的表达。
Int J Dev Biol. 2009;53(7):1105-12. doi: 10.1387/ijdb.082578dw.
2
TGFβ-1 and Wnt-3a interact to induce unique gene expression profiles in murine embryonic palate mesenchymal cells.TGFβ-1 和 Wnt-3a 相互作用诱导小鼠胚胎腭间充质细胞独特的基因表达谱。
Reprod Toxicol. 2011 Feb;31(2):128-33. doi: 10.1016/j.reprotox.2010.10.006. Epub 2010 Oct 15.
3
Expression of the retinoblastoma family of tumor suppressors during murine embryonic orofacial development.视网膜母细胞瘤家族肿瘤抑制因子在小鼠胚胎期口面部发育过程中的表达
Orthod Craniofac Res. 2003 Feb;6(1):32-47. doi: 10.1046/j.1439-0280.2003.2c035.x.
4
Neural crest-specific deletion of Ldb1 leads to cleft secondary palate with impaired palatal shelf elevation.Ldb1在神经嵴中的特异性缺失导致腭裂，同时腭突抬高受损。
BMC Dev Biol. 2014 Jan 17;14:3. doi: 10.1186/1471-213X-14-3.
5
Modulating Wnt Signaling Rescues Palate Morphogenesis in Pax9 Mutant Mice.调节Wnt信号通路可挽救Pax9突变小鼠的腭部形态发生。
J Dent Res. 2017 Oct;96(11):1273-1281. doi: 10.1177/0022034517719865. Epub 2017 Jul 10.
6
Spatio-Temporal Expression and Functional Analysis of miR-206 in Developing Orofacial Tissue.miR-206在口腔颌面部组织发育中的时空表达及功能分析
Microrna. 2019;8(1):43-60. doi: 10.2174/2211536607666180801094528.
7
Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma.成釉细胞瘤中经典型和非经典型 Wnt 配体的差异表达。
J Oral Pathol Med. 2012 Apr;41(4):332-9. doi: 10.1111/j.1600-0714.2011.01104.x. Epub 2011 Nov 14.
8
Down-regulation of Wnt10a by RNA interference inhibits proliferation and promotes apoptosis in mouse embryonic palatal mesenchymal cells through Wnt/β-catenin signaling pathway.RNA 干扰下调 Wnt10a 通过 Wnt/β-连环蛋白信号通路抑制小鼠胚胎腭中胚层细胞的增殖并促进其凋亡。
J Physiol Biochem. 2013 Dec;69(4):855-63. doi: 10.1007/s13105-013-0262-7. Epub 2013 May 28.
9
Intracellular dynamics of Smad-mediated TGFbeta signaling.Smad 介导的转化生长因子-β（TGFβ）信号通路的细胞内动力学
J Cell Physiol. 2003 Nov;197(2):261-71. doi: 10.1002/jcp.10355.
10
Rgs19 regulates mouse palatal fusion by modulating cell proliferation and apoptosis in the MEE.Rgs19 通过调节 MEE 中的细胞增殖和凋亡来调控小鼠腭裂融合。
Mech Dev. 2012 Sep-Dec;129(9-12):244-54. doi: 10.1016/j.mod.2012.07.004. Epub 2012 Jul 27.
引用本文的文献
1
Prioritization of non-coding elements involved in non-syndromic cleft lip with/without cleft palate through genome-wide analysis of mutations.通过全基因组分析突变，对非综合征型唇裂伴/不伴腭裂中涉及的非编码元件进行优先级排序。
HGG Adv. 2022 Dec 5;4(1):100166. doi: 10.1016/j.xhgg.2022.100166. eCollection 2023 Jan 12.
2
Runx2-Twist1 interaction coordinates cranial neural crest guidance of soft palate myogenesis.Runx2-Twist1 相互作用协调颅神经嵴引导软腭肌发生。
Elife. 2021 Jan 22;10:e62387. doi: 10.7554/eLife.62387.
3
Dynamic activation of Wnt, Fgf, and Hh signaling during soft palate development.腭帆张肌在软腭发育过程中动态激活 Wnt、Fgf 和 Hh 信号通路。
PLoS One. 2019 Oct 15;14(10):e0223879. doi: 10.1371/journal.pone.0223879. eCollection 2019.
4
Wnt signaling in orofacial clefts: crosstalk, pathogenesis and models.Wnt 信号在口腔面裂中的作用：串扰、发病机制和模型。
Dis Model Mech. 2019 Feb 4;12(2):dmm037051. doi: 10.1242/dmm.037051.
5
Development of Normal and Cleft Palate: A Central Role for Connective Tissue Growth Factor (CTGF)/CCN2.正常腭与腭裂的发育：结缔组织生长因子（CTGF）/CCN2的核心作用
J Dev Biol. 2018 Jul 19;6(3):18. doi: 10.3390/jdb6030018.
6
Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the β-catenin pathway.Wnt6通过β-连环蛋白途径影响小鼠胚胎腭间充质细胞的活力。
Exp Ther Med. 2017 Dec;14(6):5339-5344. doi: 10.3892/etm.2017.5240. Epub 2017 Oct 2.
7
Intersection of AHR and Wnt signaling in development, health, and disease.AHR与Wnt信号通路在发育、健康和疾病中的交集。
Int J Mol Sci. 2014 Oct 3;15(10):17852-85. doi: 10.3390/ijms151017852.
8
Down-regulation of Wnt10a by RNA interference inhibits proliferation and promotes apoptosis in mouse embryonic palatal mesenchymal cells through Wnt/β-catenin signaling pathway.RNA 干扰下调 Wnt10a 通过 Wnt/β-连环蛋白信号通路抑制小鼠胚胎腭中胚层细胞的增殖并促进其凋亡。
J Physiol Biochem. 2013 Dec;69(4):855-63. doi: 10.1007/s13105-013-0262-7. Epub 2013 May 28.
9
Epigenetic regulation of Sox4 during palate development.腭发育过程中 Sox4 的表观遗传调控
Epigenomics. 2013 Apr;5(2):131-46. doi: 10.2217/epi.13.1.
10
Chemokine-mediated migration of mesencephalic neural crest cells.趋化因子介导的中脑神经嵴细胞迁移。
Cytokine. 2011 Dec;56(3):760-8. doi: 10.1016/j.cyto.2011.09.014. Epub 2011 Oct 19.
本文引用的文献
1
Wnt11/Fgfr1b cross-talk modulates the fate of cells in palate development.Wnt11/Fgfr1b相互作用调节腭部发育中细胞的命运。
Dev Biol. 2008 Feb 15;314(2):341-50. doi: 10.1016/j.ydbio.2007.11.033. Epub 2007 Dec 7.
2
Chemical rescue of cleft palate and midline defects in conditional GSK-3beta mice.条件性 GSK-3β 小鼠腭裂和中线缺陷的化学拯救
Nature. 2007 Mar 1;446(7131):79-82. doi: 10.1038/nature05557. Epub 2007 Feb 11.
3
Wnt9b is the mutated gene involved in multifactorial nonsyndromic cleft lip with or without cleft palate in A/WySn mice, as confirmed by a genetic complementation test.经基因互补试验证实，Wnt9b是A/WySn小鼠中与伴有或不伴有腭裂的多因素非综合征性唇裂相关的突变基因。
Birth Defects Res A Clin Mol Teratol. 2006 Aug;76(8):574-9. doi: 10.1002/bdra.20302.
4
Analysis of candidate genes on chromosome 2 in oral cleft case-parent trios from three populations.对来自三个群体的口腔腭裂病例-父母三联体中2号染色体上的候选基因进行分析。
Hum Genet. 2006 Nov;120(4):501-18. doi: 10.1007/s00439-006-0235-9. Epub 2006 Sep 5.
5
Jag2-Notch1 signaling regulates oral epithelial differentiation and palate development.Jag2-Notch1信号通路调控口腔上皮分化和腭部发育。
Dev Dyn. 2006 Jul;235(7):1830-44. doi: 10.1002/dvdy.20821.
6
Expression of Wnt9b and activation of canonical Wnt signaling during midfacial morphogenesis in mice.小鼠面中部形态发生过程中Wnt9b的表达及经典Wnt信号通路的激活
Dev Dyn. 2006 May;235(5):1448-54. doi: 10.1002/dvdy.20723.
7
Regulation of Lethal giant larvae by Dishevelled.由蓬乱蛋白调控致死性巨幼虫蛋白
Nature. 2005 Oct 27;437(7063):1376-80. doi: 10.1038/nature04116.
8
Births: final data for 2003.出生情况：2003年最终数据。
Natl Vital Stat Rep. 2005 Sep 8;54(2):1-116.
9
Fate-mapping of the epithelial seam during palatal fusion rules out epithelial-mesenchymal transformation.腭融合过程中上皮缝的命运图谱排除了上皮-间充质转化。
Dev Biol. 2005 Sep 15;285(2):490-5. doi: 10.1016/j.ydbio.2005.07.027.
10
Cross-talk between the TGFbeta and Wnt signaling pathways in murine embryonic maxillary mesenchymal cells.小鼠胚胎上颌间充质细胞中转化生长因子β（TGFβ）和Wnt信号通路之间的相互作用。
FEBS Lett. 2005 Jul 4;579(17):3539-46. doi: 10.1016/j.febslet.2005.05.024.
Zotero 插件