通过血清学、组织学、免疫组织化学和聚合酶链反应测定新生儿肝内胆汁淤积症中巨细胞病毒的感染频率。

Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR.

作者信息

Bellomo-Brandao Maria Angela, Andrade Paula D, Costa Sandra C B, Escanhoela Cecilia A F, Vassallo Jose, Porta Gilda, De Tommaso Adriana M A, Hessel Gabriel

机构信息

Department of Pediatrics, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-060, Brazil.

出版信息

World J Gastroenterol. 2009 Jul 21;15(27):3411-6. doi: 10.3748/wjg.15.3411.

DOI:10.3748/wjg.15.3411

PMID:19610143

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2712903/

Abstract

AIM

To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), and to verify the relationships among these methods.

METHODS

The study comprised 101 non-consecutive infants submitted for hepatic biopsy between March 1982 and December 2005. Serological results were obtained from the patient's files and the other methods were performed on paraffin-embedded liver samples from hepatic biopsies. The following statistical measures were calculated: frequency, sensibility, specific positive predictive value, negative predictive value, and accuracy.

RESULTS

The frequencies of positive results were as follows: serology, 7/64 (11%); histological revision, 0/84; immunohistochemistry, 1/44 (2%), and PCR, 6/77 (8%). Only one patient had positive immunohistochemical findings and a positive PCR. The following statistical measures were calculated between PCR and serology: sensitivity, 33.3%; specificity, 88.89%; positive predictive value, 28.57%; negative predictive value, 90.91%; and accuracy, 82.35%.

CONCLUSION

The frequency of positive CMV varied among the tests. Serology presented the highest positive frequency. When compared to PCR, the sensitivity and positive predictive value of serology were low.

摘要

目的

通过血清学、组织学检查（寻找巨细胞）、免疫组织化学和聚合酶链反应（PCR）来确定新生儿肝内胆汁淤积症中巨细胞病毒（CMV）的感染率，并验证这些方法之间的关系。

方法

该研究纳入了1982年3月至2005年12月期间接受肝活检的101例非连续性婴儿。血清学结果来自患者病历，其他方法则对肝活检石蜡包埋的肝脏样本进行。计算了以下统计指标：感染率、敏感性、阳性预测值、阴性预测值和准确性。

结果

阳性结果的发生率如下：血清学检查，7/64（11%）；组织学检查，0/84；免疫组织化学检查，1/44（2%），PCR检查，6/77（8%）。只有1例患者免疫组织化学检查结果为阳性且PCR检查结果也为阳性。计算了PCR检查与血清学检查之间的以下统计指标：敏感性，33.3%；特异性，88.89%；阳性预测值，28.57%；阴性预测值，90.91%；准确性，82.35%。