Gomes Leonardo, Gomes Guilherme, Von Zuben Claudio J
Departamento de Biologia, 1515 UNESP - São Paulo State University, Rio Claro, SP, Brazil.
J Insect Sci. 2009;9:14. doi: 10.1673/031.009.1401.
Blowflies use discrete, ephemeral substrates for larval development. After exhaustion of the food supply, larvae will disperse in search of sites to burrow and pupate or will seek other sources of food in a process known as post-feeding larval dispersal. In this study, the effect of temperature was investigated as it is one of the most important aspects of the environmental variables in this process. 800 larvae of the blowflies Chrysomya albiceps (Wiedemann 1819) and Lucilia cuprina (Wiedemann 1830) (Diptera: Calliphoridae) were incubated in tubes covered with vermiculite at 15, 20, 25 and 30 degrees C. For each pupa, the body weight, sex and depth of burrowing were determined. Statistical tests were used to examine the relationship of depth of burrowing and body weight to the temperature at which burrowing occurred. Depth of burrowing was affected differently by temperature for both of the species studied; L. cuprina larvae burrowed deeper at lower and higher temperatures while C. albiceps larvae burrowed less at extreme temperatures. Additionally, temperature had a significant effect on the body weight of L. cuprina larvae as body weight decreased as temperature increased, whereas for C. albiceps, pupal weight increased up to 25 degrees C and then decreased abruptly at a higher temperatures. The maximum body weight was also differently affected in the two species; in L. cuprina, the maximal weight was at 15 degrees C and for C. albiceps weight was maximal at 20 degrees C.
丽蝇利用离散的、短暂的基质进行幼虫发育。食物供应耗尽后，幼虫会分散开来寻找挖掘和化蛹的地点，或者在所谓的取食后幼虫扩散过程中寻找其他食物来源。在本研究中，对温度的影响进行了调查，因为它是这个过程中环境变量最重要的方面之一。将800只白纹丽蝇（Chrysomya albiceps，维德曼，1819年）和铜绿蝇（Lucilia cuprina，维德曼，1830年）（双翅目：丽蝇科）的幼虫置于覆盖有蛭石的试管中，分别在15、20、25和30摄氏度下培养。对于每只蛹，测定其体重、性别和挖掘深度。使用统计检验来研究挖掘深度和体重与挖掘时温度之间的关系。在所研究的两个物种中，挖掘深度受温度的影响不同；铜绿蝇幼虫在较低和较高温度下挖掘得更深，而白纹丽蝇幼虫在极端温度下挖掘得较少。此外，温度对铜绿蝇幼虫的体重有显著影响，随着温度升高体重下降，而对于白纹丽蝇，蛹重直到25摄氏度时增加，然后在更高温度下突然下降。两个物种的最大体重也受到不同影响；铜绿蝇的最大体重出现在15摄氏度，白纹丽蝇的最大体重出现在20摄氏度。
