Youngnak-Piboonratanakit Pornpan, Dhanuthai Kittipong, Thongprasom Kobkan, Luckprom Pimporn, Sarideechaigul Wilairat, Luangjarmekorn Lakana, Azuma Miyuki
Department of Oral Medicine, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
J Oral Pathol Med. 2009 Oct;38(9):689-94. doi: 10.1111/j.1600-0714.2009.00805.x. Epub 2009 Jul 15.
Oral lichen planus (OLP) is a common chronic inflammatory mucosal disease in which T-cell-mediated immune responses are implicated in the pathogenesis. The purpose of this study was to investigate the effect of 0.1% fluocinolone acetonide in orabase (FAO) on the in situ expression of IFN-gamma in patients with OLP.
Twenty OLP patients were enrolled in this study. Biopsy specimens and serum samples were obtained before and 1-month after the treatment with 0.1% FAO. In situ expression and serum levels of IFN-gamma were determined using immunohistochemistry and ELISA, respectively.
The number of IFN-gamma-positive mononuclear cells in OLP lesions before the treatment was significantly higher as compared with that after the treatment. Similarly, the mean number of total mononuclear cells was clearly decreased after the treatment. However, the serum levels of IFN-gamma were not detectable.
Our results suggest that IFN-gamma expression in OLP tissue may involve in the immunopathogenesis and the treatment with 0.1% FAO had an immunomodulating effect on the decrease of IFN-gamma.
口腔扁平苔藓（OLP）是一种常见的慢性炎症性黏膜疾病，其发病机制涉及T细胞介导的免疫反应。本研究旨在探讨0.1%丙酮化氟轻松（FAO）口腔糊剂对OLP患者干扰素-γ原位表达的影响。
本研究纳入20例OLP患者。在使用0.1% FAO治疗前及治疗1个月后获取活检标本和血清样本。分别采用免疫组织化学和酶联免疫吸附测定法测定干扰素-γ的原位表达和血清水平。
治疗前OLP病损中干扰素-γ阳性单核细胞数量显著高于治疗后。同样，治疗后总单核细胞的平均数量明显减少。然而，未检测到血清干扰素-γ水平。
我们的结果表明，OLP组织中干扰素-γ的表达可能参与免疫发病机制，且0.1% FAO治疗对干扰素-γ的降低具有免疫调节作用。
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