Instituto de Biología y Genética Molecular, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas, Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), C/Sanz y Forés s/n, E-47003 Valladolid, Spain.
Mech Dev. 2009 Oct;126(10):873-81. doi: 10.1016/j.mod.2009.07.004. Epub 2009 Jul 18.
Several members of the FGF gene family have been shown to intervene from various tissue sources to direct otic placode induction and otic vesicle formation. In this study we define the roles of FGF8, found in different expression domains during this process, in mice and chickens. By conditional inactivation of Fgf8 in distinct tissue compartments we demonstrate that Fgf8 is required in the mesoderm and endoderm during early inner ear development. In the chicken embryo, overexpression of Fgf8 from various tissue sources during otic specification leads to a loss of otic tissue. In contrast ectopic overexpression of Fgf10, a major player during murine otic induction, does not influence otic vesicle formation in chicken embryos but results in the formation of ectopic structures with a non-otic character. This study underlines the crucial role of a defined Fgf8 expression pattern controlling inner ear formation in vertebrates.
已经有研究表明，成纤维细胞生长因子（FGF）基因家族的几个成员可以从各种组织来源介入，指导耳原基诱导和耳泡形成。在这项研究中，我们在小鼠和鸡中定义了 FGF8 的作用，该因子在这个过程的不同表达域中被发现。通过在特定组织隔室中条件性灭活 Fgf8，我们证明 Fgf8 在早期内耳发育过程中在内胚层和中胚层中是必需的。在鸡胚胎中，在耳原基形成期间，来自各种组织来源的 Fgf8 的异位过表达导致耳组织丧失。相比之下，在小鼠耳诱导过程中起主要作用的 Fgf10 的异位过表达不会影响鸡胚胎中耳泡的形成，但会导致具有非耳特征的异位结构的形成。这项研究强调了一个明确的 Fgf8 表达模式在控制脊椎动物内耳形成中的关键作用。
