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Finding better protein engineering strategies.

作者信息

Kazlauskas Romas J, Bornscheuer Uwe T

机构信息

Biotechnology Institute and Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, St. Paul, Minnesota, USA.

出版信息

Nat Chem Biol. 2009 Aug;5(8):526-9. doi: 10.1038/nchembio0809-526.

DOI:10.1038/nchembio0809-526

PMID:19620988

Abstract

摘要

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Finding better protein engineering strategies.

Nat Chem Biol. 2009 Aug;5(8):526-9. doi: 10.1038/nchembio0809-526.

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Biotechnol J. 2007 Feb;2(2):180-91. doi: 10.1002/biot.200600170.

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Curr Opin Biotechnol. 2000 Aug;11(4):319-24. doi: 10.1016/s0958-1669(00)00106-3.

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本文引用的文献

Directed enzyme evolution via small and effective neutral drift libraries.

Nat Methods. 2008 Nov;5(11):939-42. doi: 10.1038/nmeth.1262. Epub 2008 Oct 19.

Constructing and analyzing the fitness landscape of an experimental evolutionary process.

Chembiochem. 2008 Sep 22;9(14):2260-7. doi: 10.1002/cbic.200800371.

Addressing the numbers problem in directed evolution.

Chembiochem. 2008 Jul 21;9(11):1797-804. doi: 10.1002/cbic.200800298.

Selection and evolution of enzymes from a partially randomized non-catalytic scaffold.

Nature. 2007 Aug 16;448(7155):828-31. doi: 10.1038/nature06032.

Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.

Biol Direct. 2007 Jun 28;2:17. doi: 10.1186/1745-6150-2-17.

Improving catalytic function by ProSAR-driven enzyme evolution.

Nat Biotechnol. 2007 Mar;25(3):338-44. doi: 10.1038/nbt1286. Epub 2007 Feb 18.

Darwinian evolution can follow only very few mutational paths to fitter proteins.

Science. 2006 Apr 7;312(5770):111-4. doi: 10.1126/science.1123539.

Protein stability promotes evolvability.

Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5869-74. doi: 10.1073/pnas.0510098103. Epub 2006 Mar 31.

Bioethanol.

Curr Opin Chem Biol. 2006 Apr;10(2):141-6. doi: 10.1016/j.cbpa.2006.02.035. Epub 2006 Mar 7.

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space.

Angew Chem Int Ed Engl. 2006 Feb 13;45(8):1236-41. doi: 10.1002/anie.200502746.

Finding better protein engineering strategies.

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