酶联免疫吸附测定法和放射免疫测定技术在测定妇科患者血清胎盘蛋白14水平中的比较研究

Comparative study of enzyme-linked immunosorbent assay and radioimmunoassay techniques in determining serum placental protein 14 levels in gynecologic patients.

作者信息

Scherbakova L A, Göcze P M, Olefirenko G A, Than G N, Szabò D G, Petrunin D D, Tatarinov Y S, Csaba I F

机构信息

Department of Biochemistry, Second Medical Institute, Moscow, USSR.

出版信息

Tumour Biol. 1991;12(5):267-71. doi: 10.1159/000217714.

DOI:10.1159/000217714

PMID:1962148

Abstract

A comparative analysis of radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques in determining placental protein 14 (PP14) levels in gynecologic patients was performed. Serum PP14 levels were assessed in the patients in the last period of gestation, during menopause and in healthy nonpregnant controls. The possible discrepancy in PP14 levels was also examined in gynecologic patients with benign and malignant tumors. Both RIA and ELISA techniques proved to be sufficiently sensitive to measure PP14 concentration.

摘要

对放射免疫测定法（RIA）和酶联免疫吸附测定法（ELISA）在测定妇科患者胎盘蛋白14（PP14）水平方面进行了比较分析。对妊娠末期患者、绝经期间患者以及健康非妊娠对照组的血清PP14水平进行了评估。还对患有良性和恶性肿瘤的妇科患者PP14水平的可能差异进行了检查。结果证明，RIA和ELISA技术在测量PP14浓度方面都具有足够的灵敏度。