Lu Guang-Hua, Wang Chao, Sun Zhe
Key Lab for Hydrology-Water Resources and Hydraulic Engineering, Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, College of Environmental Science and Engineering, Hohai University, Jiangsu, China.
Biomed Environ Sci. 2009 Apr;22(2):112-7. doi: 10.1016/S0895-3988(09)60032-5.
To isolate, incubate, and identify 4-chlorophenol-degrading complex bacteria, determine the tolerance of these bacteria to phenolic derivatives and study their synergetic metabolism as well as the aboriginal micrpbes and co-metabolic degradation of mixed chlorophenols in river water.
Microbial community of complex bacteria was identified by plate culture observation techniques and Gram stain method. Bacterial growth inhibition test was used to determine the tolerance of complex bacteria to toxicants. Biodegradability of phenolic derivatives was determined by adding 4-chlorophenol-degrading bacteria in river water.
The complex bacteria were identified as Mycopiana, Alcaligenes, Pseudomonas, and Flavobacterium. The domesticated complex bacteria were more tolerant to phenolic derivatives than the aboriginal bacteria from Qinhuai River. The biodegradability of chlorophenols, dihydroxybenzenes and nitrophenols under various aquatic conditions was determined and compared. The complex bacteria exhibited a higher metabolic efficiency on chemicals than the aboriginal microbes, and the final removal rate of phenolic derivatives was increased at least by 55% when the complex bacteria were added into river water. The metabolic relationship between dominant mixed bacteria and river bacteria was studied.
The complex bacteria domesticated by 4-chlorophenol can grow and be metabolized to take other chlorophenols, dihydroxybenzenes and nitrophenols as the sole carbon and energy source. There is a synergetic metabolism of most compounds between the aboriginal microbes in river water and the domesticated complex bacteria. 4-chlorophenol-degrading bacteria can co-metabolize various chlorophenols in river water.
分离、培养和鉴定降解4-氯酚的复合菌，测定这些细菌对酚类衍生物的耐受性，研究其协同代谢以及河水中土著微生物对混合氯酚的共代谢降解情况。
采用平板培养观察技术和革兰氏染色法鉴定复合菌的微生物群落。通过细菌生长抑制试验测定复合菌对毒物的耐受性。在河水中添加降解4-氯酚的细菌来测定酚类衍生物的生物降解性。
复合菌被鉴定为枝顶孢属、产碱菌属、假单胞菌属和黄杆菌属。驯化后的复合菌比秦淮河的土著细菌对酚类衍生物具有更高的耐受性。测定并比较了不同水生条件下氯酚、二羟基苯和硝基酚的生物降解性。复合菌对化学物质的代谢效率高于土著微生物，向河水中添加复合菌后，酚类衍生物的最终去除率至少提高了55%。研究了优势混合菌与河水中细菌的代谢关系。
经4-氯酚驯化的复合菌能够生长并进行代谢，以其他氯酚、二羟基苯和硝基酚作为唯一碳源和能源。河水中的土著微生物与驯化后的复合菌对大多数化合物存在协同代谢作用。降解4-氯酚的细菌能够对河水中的各种氯酚进行共代谢。
