Yu Hua-peng, Sun Jun-yong, Wu Lian, Xu Hong-wei, Wang Lun, Zhou Yun-you
Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Apr;29(4):1041-4.
The characteristics of host-guest complexation between cucurbit [7] uril (CB7) and methylene blue (MB) were investigated by fluorescence spectrometry in acetate buffer solution at room temperature. It was found that the fluorescence intensity of methylene blue regularly increased upon the addition of cucurbit [7] uril accompanying with a blue-shift of the position of the emission maximum. The results indicate the formation of complex between CB7 and MB at a 1 : 1 complex stoichiometry and the association constant was calculated by applying a deduced equation. 1H NMR spectra were applied to to complement the fluorescence work to verify the formation of the complex. From the temperature dependence of the equilibrium constants, thermodynamics parametersH andS values were obtained, indicating an enthalpic driving force for complexation. The possible interaction mechanism was also discussed. This work may extend the application range of cucurbit [7] uril in biochemistry and pharmaceutical analysis.
在室温下，于醋酸盐缓冲溶液中采用荧光光谱法研究了葫芦[7]脲（CB7）与亚甲基蓝（MB）之间的主客体络合特性。结果发现，加入葫芦[7]脲后，亚甲基蓝的荧光强度有规律地增强，同时发射峰位置发生蓝移。结果表明CB7与MB以1:1的化学计量比形成络合物，并通过推导方程计算了缔合常数。采用1H NMR光谱对荧光研究进行补充，以验证络合物的形成。根据平衡常数的温度依赖性，获得了热力学参数ΔH和ΔS值，表明络合过程存在焓驱动力。还讨论了可能的相互作用机制。这项工作可能会扩展葫芦[7]脲在生物化学和药物分析中的应用范围。
