Li Lijun, Lu Fuping, Zhang Min, Guo Yaping, Ma Enbo
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China.
J Environ Sci (China). 2009;21(4):509-13. doi: 10.1016/s1001-0742(08)62300-1.
The goal of the present study was to compare the different survival of Oxya chinensis exposed to chromium(VI) among allozyme genotypes to gain a better understanding of the relationship between the genetic variations and environmental disturbance. This study analyzed the occurrence of genotypes in O. chinensis population exposed to Cr(VI). O. chinensis samples were collected at Yuanping, Shanxi Province, China and used in acute toxicity tests. Specimens were assigned to Cr(VI) exposure (LD50: 291.0 mg/kg) for 24 h. The genetic composition of both dead and survived specimens was analyzed with horizontal starch gel electrophoresis in four enzymes (GPI, PGM, LDH, and ME). The results indicated that under Cr(VI) exposure, specimens with different genotypes had various mortalities at the four loci in laboratory conditions, and there was a genetic basis for tolerance in O. chinensis during acute exposure to Cr(VI).
本研究的目的是比较不同等位酶基因型的中华稻蝗在暴露于六价铬时的不同存活率,以便更好地理解遗传变异与环境干扰之间的关系。本研究分析了暴露于六价铬的中华稻蝗种群中基因型的出现情况。在中国山西省原平采集中华稻蝗样本,并用于急性毒性试验。将样本分配到六价铬暴露组(半数致死剂量:291.0毫克/千克),暴露24小时。用水平淀粉凝胶电泳分析四种酶(葡萄糖磷酸异构酶、磷酸葡萄糖变位酶、乳酸脱氢酶和苹果酸酶)在死亡和存活样本中的遗传组成。结果表明,在六价铬暴露下,在实验室条件下,不同基因型的样本在四个位点具有不同的死亡率,中华稻蝗在急性暴露于六价铬期间存在耐受的遗传基础。