State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 FengTai Dongdajie Street, Beijing 100071, PR China.
Vaccine. 2009 Dec 9;27(52):7436-41. doi: 10.1016/j.vaccine.2009.07.015. Epub 2009 Jul 25.
The protective antigen (PA) of Bacillus anthracis and the Fraction 1 Capsular Antigen (F1 antigen), V antigen of Yersinia pestis have been demonstrated to be potential immunogens and candidate vaccine sub-units against anthrax and plague respectively. In this study, the authors have investigated the antibody responses and the protective efficacy when the antigens were administered separately or in combination intramuscularly formulation adsorbed to an aluminum hydroxide adjuvant. Results show that immunized rF1 + rV and rPA antigen together was as effective as separately for induction of serological antibody response, and these titers were maintained for over 1 year in mice. An isotype analysis of the serum indicates that the co-administration of these antigens did not influence the antigen-specific IgG1/IgG2a ratio which was consistent with a Th2 bias. Furthermore, the combined vaccine comprising the protein antigens rF1 + rV + rPA has been demonstrated to protect mice from subcutaneous challenge with 10(7) colony-forming units (CFU) virulent Y. pestis strain, and to fully protect rabbit against subcutaneous challenge with 1.2x10(5) colony-forming units (CFU) virulent B. anthracis spores. These data show that the protective efficacy was unaffected when the antigens were administered in combination.
炭疽杆菌保护性抗原（PA）和 1 型荚膜抗原（F1 抗原）、鼠疫耶尔森氏菌 V 抗原已被证明分别是炭疽和鼠疫的潜在免疫原和候选疫苗亚单位。在这项研究中，作者研究了将抗原分别或联合以肌肉内制剂形式与氢氧化铝佐剂吸附给药时的抗体反应和保护效力。结果表明，rF1+rV 和 rPA 抗原一起免疫与单独免疫一样有效诱导血清抗体反应，并且这些滴度在小鼠中维持了 1 年以上。血清的同种型分析表明，这些抗原的联合给药并不影响抗原特异性 IgG1/IgG2a 比值，这与 Th2 偏向一致。此外，包含蛋白抗原 rF1+rV+rPA 的联合疫苗已被证明可保护小鼠免受 10（7）菌落形成单位（CFU）毒力鼠疫耶尔森氏菌菌株的皮下攻击，并可完全保护兔免受 1.2x10（5）菌落形成单位（CFU）毒力炭疽芽孢杆菌孢子的皮下攻击。这些数据表明，当抗原联合给药时，保护效力不受影响。
