Wang Yanju, Lin Wei-Yu, Liu Kan, Lin Rachel J, Selke Matthias, Kolb Hartmuth C, Zhang Nangang, Zhao Xing-Zhong, Phelps Michael E, Shen Clifton K F, Faull Kym F, Tseng Hsian-Rong
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, building 114, Los Angeles, CA 90095, USA.
Lab Chip. 2009 Aug 21;9(16):2281-5. doi: 10.1039/b907430a. Epub 2009 Jun 17.
An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.
已经开发出一种集成微流控装置,以牛碳酸酐酶II(bCAII)点击化学系统作为概念验证研究,并行进行1024个原位点击化学反应,并采用一种使用固相萃取(SPE)纯化和电喷雾电离质谱、多反应监测(MRM)分析的快速命中鉴定方法,所有这些都提高了下游分析的灵敏度和通量。