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[嫁接对低温弱光下甜椒幼苗光合作用的影响]

[Effects of grafting on photosynthesis of sweet pepper seedlings under low temperature and weak light intensity].

作者信息

Zheng Nan, Wang Mei-ling, Wang Hong-tao, Ai Xi-zhen

机构信息

Agriculture Ministry Key Laboratory of Horticultural Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai' an 271018, Shandong, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2009 Mar;20(3):591-6.

Abstract

Sweet pepper 'Chifengtexuan' seedlings were grafted onto 'Weishi' and 'Buyeding' rootstocks, treated with low temperature (8 degrees C/ 5 degrees C) and weak light intensity (100 micromol x m(-2) s(-1)) for 7 days, and then recovered under normal conditions (25 degrees C/ 18 degrees C, PFD 550-600 micromol x s(-1)) for 3 days to study the variations of their gas exchange parameters, carboxylation efficiency, and fluorescence parameters, with the own-rooted 'Chifengtexuan' seedlings as the control. The results showed that on the 3rd day of low temperature and weak light intensity stress, the photosynthetic rate (Pn), stomatal conductance (Gs), and carboxylation efficiency (CE) of both own-rooted and grafted seedling leaves decreased more than 50%, and after then, the Pn and Gs tended to stable while CE decreased continually. The intercellular CO2 concentration (Ci) declined first, but enhanced after the 4th day of the stress. Low temperature and weak light intensity decreased the maximal photochemical efficiency of PS II in darkness (Fv/Fm), actual photochemical efficiency of PS II during illumination (phi (PS II)) and electron transport rate (ETR), but increased the initial fluorescence (Fo), which implied that the stress caused definite damage of photosynthetic apparatus. However, the damage diminished after 3 days of recovery. Comparing with those of own-rooted seedlings, the Pn, Gs, CE, phi(PS II), and Fv/Fm of grafted seedlings increased to various degrees, and Fo decreased markedly during the stress. It was concluded that grafting could promote the photosynthetic function, and alleviate the effects of low temperature and weak light intensity on the photosynthesis of sweet pepper seedlings.

摘要

将甜椒‘赤峰特选’幼苗嫁接在‘卫士’和‘布野丁’砧木上,在低温(8℃/5℃)和弱光强度(100 μmol·m⁻²·s⁻¹)条件下处理7天,然后在正常条件(25℃/18℃,光合光子通量密度550 - 600 μmol·s⁻¹)下恢复3天,以自根‘赤峰特选’幼苗为对照,研究其气体交换参数、羧化效率和荧光参数的变化。结果表明,在低温弱光胁迫第3天,自根和嫁接幼苗叶片的光合速率(Pn)、气孔导度(Gs)和羧化效率(CE)均下降超过50%,之后Pn和Gs趋于稳定,而CE持续下降。胞间CO₂浓度(Ci)先下降,但在胁迫第4天后升高。低温弱光降低了PS II在黑暗中的最大光化学效率(Fv/Fm)、光照下PS II的实际光化学效率(φ(PS II))和电子传递速率(ETR),但增加了初始荧光(Fo),这表明胁迫对光合机构造成了一定损伤。然而,恢复3天后损伤减轻。与自根幼苗相比,嫁接幼苗的Pn、Gs、CE、φ(PS II)和Fv/Fm在胁迫期间有不同程度的增加,Fo显著降低。得出结论:嫁接可促进光合功能,减轻低温弱光对甜椒幼苗光合作用的影响。

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