Institute of Physiology and Pathophysiology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
J Cereb Blood Flow Metab. 2009 Dec;29(12):1879-84. doi: 10.1038/jcbfm.2009.103. Epub 2009 Jul 29.
The blood-brain barrier (BBB) closely interacts with the neuronal parenchyma in vivo. To replicate this interdependence in vitro, we established a murine coculture model composed of brain endothelial cell (BEC) monolayers with cortical organotypic slice cultures. The morphology of cell types, expression of tight junctions, formation of reactive oxygen species, caspase-3 activity in BECs, and alterations of electrical resistance under physiologic and pathophysiological conditions were investigated. This new BBB model allows the application of techniques such as laser scanning confocal microscopy, immunohistochemistry, fluorescent live cell imaging, and electrical cell substrate impedance sensing in real time for studying the dynamics of BBB function under defined conditions.
血脑屏障（BBB）与体内的神经元实质密切相互作用。为了在体外复制这种相互依存关系，我们建立了一个由脑内皮细胞（BEC）单层和皮质器官型切片培养物组成的小鼠共培养模型。研究了细胞类型的形态、紧密连接的表达、活性氧的形成、BEC 中的半胱天冬酶-3 活性以及在生理和病理生理条件下电阻的变化。这种新的 BBB 模型允许应用激光扫描共聚焦显微镜、免疫组织化学、荧光活细胞成像和电细胞底物阻抗传感等技术，实时研究特定条件下 BBB 功能的动力学。
