International Center for Spinal Cord Injury, Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, Maryland, USA.
J Neurotrauma. 2009 Dec;26(12):2313-22. doi: 10.1089/neu.2009.0987.
Bone marrow stromal cells (BMSC) transplanted into the contused spinal cord may support repair by improving tissue sparing. We injected allogeneic BMSC into the moderately contused adult rat thoracic spinal cord at 15 min (acute) and at 3, 7, and 21 days (delayed) post-injury and quantified tissue sparing and BMSC survival up to 4 weeks post-transplantation. BMSC survival within the contusion at 7 days post-transplantation was significantly higher with an acute injection (32%) and 3-day delayed injection (52%) than with a 7- or 21-day delayed injection (9% both; p < 0.01). BMSC survival at 28 days post-transplantation was close to 0 in all paradigms, indicating rejection. In contused rats without a BMSC transplant (controls), the volume of spared tissue gradually decreased until 46% (p < 0.001) of the volume of a comparable uninjured spinal cord segment at 49 days post-injury. In rats with BMSC, injected at 15 min, 3, or 7 days post-injury, spared tissue volume was significantly higher in grafted rats than in control rats at the respective endpoints (i.e., 28, 31, and 35 days post-injury). Acute and 3-day delayed but not 7- and 21-day delayed injection of BMSC significantly improved tissue sparing, which was strongly correlated (r = 0.79-1.0) to BMSC survival in the first week after injection into the contusion. Our data showed that neuroprotective effects of BMSC transplanted into a moderate rat spinal cord contusion depend strongly on their survival during the first week post-injection. Acutely injected BMSC elicit more tissue sparing than delayed injected BMSC.
骨髓基质细胞（BMSC）移植到挫伤的脊髓中可能通过改善组织保留来支持修复。我们在损伤后 15 分钟（急性）和 3、7 和 21 天（延迟）将同种异体 BMSC 注射到中度挫伤的成年大鼠胸段脊髓中，并在移植后 4 周内定量评估组织保留和 BMSC 存活情况。与 7 天或 21 天延迟注射（均为 9%；p < 0.01）相比，急性注射（32%）和 3 天延迟注射（52%）时，移植后 7 天 BMSC 在挫伤部位的存活明显更高。移植后 28 天，所有方案中 BMSC 的存活都接近 0，表明发生了排斥反应。在没有 BMSC 移植（对照）的挫伤大鼠中，在损伤后 49 天，保留组织的体积逐渐减少，直到与未受伤脊髓节段相比减少了 46%（p < 0.001）。在损伤后 15 分钟、3 天或 7 天注射 BMSC 的大鼠中，与对照大鼠相比，在各自的时间点（即损伤后 28、31 和 35 天），移植大鼠的保留组织体积明显更高。急性和 3 天延迟但不是 7 天和 21 天延迟注射 BMSC 可显著改善组织保留，这与注射后第 1 周 BMSC 在挫伤中的存活呈强相关（r = 0.79-1.0）。我们的数据表明，移植到中度大鼠脊髓挫伤中的 BMSC 的神经保护作用强烈依赖于其在注射后第 1 周内的存活。急性注射的 BMSC 比延迟注射的 BMSC 产生更多的组织保留。
