Nolen-Walston R D, Kuehn H, Boston R C, Mazan M R, Wilkins P A, Bruns S, Hoffman A M
Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA 19348, USA.
J Vet Intern Med. 2009 May-Jun;23(3):631-5. doi: 10.1111/j.1939-1676.2009.0307.x.
Inflammatory airway disease has a high prevalence in horses, but is often a diagnostic challenge. Flowmetric plethysmography and histamine bronchoprovocation (FP/HBP) is a simple and effective tool for diagnosis, but reproducibility of these measurements made over time has not been established.
We hypothesize that the measurement of airway responsiveness in horses using FP/HBP is consistent over both short and long periods of time.
Twenty-nine healthy adult horses from 2 university herds.
In this prospective experimental study, airway responsiveness was determined in each horse at day 0 (baseline [BL]) with FP/ HBP, using PC35 (provocative concentration of histamine needed to increase Delta(flow) by 35%) as a measure of airway responsiveness. Each horse was re-tested 1-4 weeks after BL (short-term [ST]) and again at 3-12 months after BL (long-term [LT]).
In the ST period, 23/27 (85%) of the horses had a PC35 that was within 1 doubling concentration of histamine of their BL value, with a mean change of 0.52 doubling concentrations (95% CI 0.26-0.79, range 0-2.06). For the LT data, 19/26 (73%) of horses were within 1 doubling concentration of their BL value, with a mean change of 0.81 doubling concentrations (95% CI 0.45-1.17, range 0.14-3.10). There was no significant difference in reproducibility between the 2 groups of subjects.
Repeated measurements of airway responsiveness obtained with FP/HBP show acceptable reproducibility over time periods up to a year. However, caution must be used when testing horses when ambient air temperature is low.
炎症性气道疾病在马匹中患病率很高，但通常是一个诊断难题。流量容积描记术和组胺支气管激发试验（FP/HBP）是一种简单有效的诊断工具，但这些测量值随时间的可重复性尚未得到证实。
我们假设使用FP/HBP测量马匹气道反应性在短期和长期内都是一致的。
来自两所大学马群的29匹健康成年马。
在这项前瞻性实验研究中，于第0天（基线[BL]）对每匹马使用FP/HBP测定气道反应性，使用PC35（使流量变化Δ(flow)增加35%所需的组胺激发浓度）作为气道反应性的指标。每匹马在BL后1 - 4周（短期[ST]）再次进行测试，并在BL后3 - 12个月（长期[LT]）再次测试。
在短期阶段，23/27（85%）的马匹PC35值在其BL值的1个组胺加倍浓度范围内，平均变化为0.52个加倍浓度（95%可信区间0.26 - 0.79，范围0 - 2.06）。对于长期数据，19/26（73%）的马匹在其BL值的1个加倍浓度范围内，平均变化为0.81个加倍浓度（95%可信区间0.45 - 1.17，范围0.14 - 3.10）。两组受试者之间的可重复性没有显著差异。
使用FP/HBP重复测量气道反应性在长达一年的时间段内显示出可接受的可重复性。然而，在环境空气温度较低时对马匹进行测试时必须谨慎。
