链霉菌属自我抵抗机制：多柔比星增效的 drrA、drrB 和 drrC 的过表达。
Self-resistance mechanism in Streptomyces peucetius: overexpression of drrA, drrB and drrC for doxorubicin enhancement.
机构信息
Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, SunMoon University, # 100 Kalsan-ri, Asansi, Chungnam 336-708, Republic of Korea.
出版信息
Microbiol Res. 2010 May 30;165(4):259-67. doi: 10.1016/j.micres.2009.04.002. Epub 2009 Aug 3.
The resistance genes drrABC from Streptomyces peucetius ATCC 27952 were cloned into the pIBR25 expression vector under a strong ermE* promoter to enhance doxorubicin (DXR) production. The recombinant expression plasmids, pDrrAB25, pDrrC25 and pDrrABC25, were constructed to overexpress drrAB, drrC and drrABC, respectively, in S. peucetius ATCC 27952. The recombinant strains produced more DXR than the parental strain: a 2.2-fold increase with pDrrAB25, a 5.1-fold increase with pDrrC25, and a 2.4-fold increase with pDrrABC25. We also studied the relative ratios of doxorubicin, daunorubicin and epsilon-rhodomycinone produced in these recombinant strains.
来自链霉菌 peucetius ATCC 27952 的抗性基因 drrABC 被克隆到 pIBR25 表达载体中，在一个强 ermE*启动子的控制下，以增强多柔比星（DXR）的产量。构建了重组表达质粒 pDrrAB25、pDrrC25 和 pDrrABC25，分别在链霉菌 peucetius ATCC 27952 中过表达 drrAB、drrC 和 drrABC。重组菌株比亲本菌株产生了更多的 DXR：pDrrAB25 增加了 2.2 倍，pDrrC25 增加了 5.1 倍，pDrrABC25 增加了 2.4 倍。我们还研究了这些重组菌株中产生的多柔比星、柔红霉素和 ε-罗红霉素的相对比例。
Zotero 插件