Malik Faraz Arshad, Sanders Andrew J, Kayani Mahmood A, Jiang Wen G
Metastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Cardiff, Wales, United Kingdom.
Cancer Genomics Proteomics. 2009 Jul-Aug;6(4):205-13.
KAI1, also known as CD82, is a candidate metastasis suppressor gene and has been indicated in the disease progression of certain solid tumours, including those of breast cancer. The present study aimed to investigate the importance of KAI1 as a potential metastasis suppressor in breast cancer cells. MDA-MB-231 and MCF-7 sublines with different patterns of KAI1 expression were created by way of anti-KAI1 transgene or transfection of KAI1 expression construct. Cell adhesion was markedly increased in cancer cells showing increased expression of KAI1 (MCF-7(KAI1EXP), p=0.021 vs. control cells), while it was significantly reduced in the KAI1 knockout subline, MDA-MB-231(KAI1KO) (p=0.002 and 0.0004, respectively). Significant increase of cell migration of MCF-7(KAI1EXP) cells (p=0.024 vs. control) and restricted motility of MDA-MB-231(KAI1KO) cells (p=0.003) were observed. Furthermore, MCF-7(KAI1EXP) cells also showed reduced cell invasion (p=0.022), while MDA-MB-231(KAI1KO) cell line showed a significant increase in invasion (p=0.0063 and p=0.007, respectively). KAI1 did not affect cell growth. It is concluded therefore that KAI1 plays an important role in cell adhesion, invasion and migration of breast cancer cells, in vitro, and is a potential metastasis suppressor gene in breast cancer.
KAI1，也被称为CD82，是一种候选转移抑制基因，已被证明在某些实体瘤（包括乳腺癌）的疾病进展中发挥作用。本研究旨在探讨KAI1作为乳腺癌细胞潜在转移抑制因子的重要性。通过抗KAI1转基因或转染KAI1表达构建体，创建了具有不同KAI1表达模式的MDA-MB-231和MCF-7亚系。在KAI1表达增加的癌细胞（MCF-7(KAI1EXP)，与对照细胞相比，p=0.021）中，细胞黏附显著增加，而在KAI1敲除亚系MDA-MB-231(KAI1KO)中，细胞黏附则显著降低（分别为p=0.002和0.0004）。观察到MCF-7(KAI1EXP)细胞的细胞迁移显著增加（与对照相比，p=0.024），而MDA-MB-231(KAI1KO)细胞的运动受到限制（p=0.003）。此外，MCF-7(KAI1EXP)细胞的细胞侵袭也减少（p=0.022），而MDA-MB-231(KAI1KO)细胞系的侵袭则显著增加（分别为p=0.0063和p=0.007）。KAI1不影响细胞生长。因此得出结论，KAI1在体外对乳腺癌细胞的细胞黏附、侵袭和迁移起着重要作用，是乳腺癌中一种潜在的转移抑制基因。
