斜纹夜蛾普通夜蛾幼虫中游离氧有效掺入到volicitin中。
Efficient incorporation of free oxygen into volicitin in Spodoptera litura common cutworm larvae.
作者信息
Ishikawa Chihiro, Yoshinaga Naoko, Aboshi Takako, Nishida Ritsuo, Mori Naoki
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
出版信息
Biosci Biotechnol Biochem. 2009 Aug;73(8):1883-5. doi: 10.1271/bbb.90207. Epub 2009 Aug 7.
Volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] has previously been identified from the lepidopteran larval regurgitant as an elicitor of plant volatile emission. The efficient incorporation of free oxygen into volicitin by Spodoptera litura larvae is demonstrated here by rearing them under (18)O(2) for three days. (18)O-labeling of the hydroxyl group of volicitin was confirmed by liquid chromatography/mass spectrometry-ion trap-time-of-flight (LC/MS-IT-TOF) and suggests the activity of a monooxygenase in volicitin biosynthesis.
volicitin [N-(17-羟基亚麻酰基)-L-谷氨酰胺] 先前已从鳞翅目幼虫的反刍液中鉴定出来，是植物挥发性物质释放的诱导剂。本文通过在(18)O(2)条件下饲养斜纹夜蛾幼虫三天，证明了斜纹夜蛾幼虫能将游离氧有效掺入volicitin中。通过液相色谱/质谱-离子阱-飞行时间 (LC/MS-IT-TOF) 确认了volicitin羟基的(18)O标记，这表明单加氧酶在volicitin生物合成中具有活性。
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