Bai Xiao-Hui, Cai Yun-Long, Zhou Bin-Hui, Zhi Xing-Hua
Laboratory of Water Science and Technology, College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China.
Huan Jing Ke Xue. 2009 Jun 15;30(6):1649-52.
The growth of microbe and formation of biofilm in water distribution system were important factors affecting the security of water quality. The number of ammonia-oxidizing bacteria (AOB) in biofilm of a chloraminated drinking water distribution system in Shanghai was detected by MPN-Griess method, and the relations among AOB, nitrification and chloraminated disinfection were analyzed. Meanwhile, the effects of AOB on chloraminated disinfection fastness and attenuation by simulation experiment were studied. The result indicated that the number of ammonia-oxidizing bacteria in pipe biofilm was between 1.0 x 10(2)-4.3 x 10(5) MPN/g dry biofilm. Correlation coefficients of AOB with ammonia, nitrite and nitrate were -0.563, 0.603 and -0.563. Correlation coefficients of AOB with total chlorine and mono-chloramine were -0.659 and -0.571. Fastness of AOB to chloramine was higher than heterotrophic bacteria and AOB can deplete more chloramine than HPC.
微生物在供水系统中的生长及生物膜的形成是影响水质安全的重要因素。采用MPN-格里斯法检测了上海某采用氯胺消毒的饮用水供水系统生物膜中氨氧化细菌（AOB）的数量，并分析了AOB、硝化作用与氯胺消毒之间的关系。同时，通过模拟实验研究了AOB对氯胺消毒持久性及衰减的影响。结果表明，管道生物膜中氨氧化细菌数量在1.0×10²-4.3×10⁵MPN/g干生物膜之间。AOB与氨、亚硝酸盐和硝酸盐的相关系数分别为-0.563、0.603和-0.563。AOB与总氯和一氯胺的相关系数分别为-0.659和-0.571。AOB对氯胺的耐受性高于异养细菌，且AOB比异养平板计数法（HPC）消耗更多的氯胺。
