[玻璃化溶液中添加川芎嗪对同种异体周围神经移植再生的影响]

Chen Zenggang, Jiang Dianming, Ou Yunsheng, Huang Yingru, Yuan Xin, Liu Jianwen

Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, P.R. China.

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Jul;23(7):868-72.

OBJECTIVE

To investigate the effect of tetramethylpyrazine (TMP) with a certain concentration added to vitrification solution on peripheral nerve allografts regeneration.

METHODS

Forty-eight healthy clean SD male rats were selected as donors, and 96 healthy clean Wistar male rats as recipients, all rats being 3 months old and weighing 200-250 g. The sciatic nerves segments of 15 mm were removed from the donors, then randomly divided into 4 groups according to vitrification solution containing TMP. No TMP was used in group A as the control group; 100 mg/L, 200 mg/L and 400 mg/L TMP were used in group B, group C and group D, respectively. Then them were cryo-preserved at -196 degrees C for 3 weeks. Nerve defect of 10 mm in length was made in the sciatic nerves of recipients. After rewarming, the allografts were transplanted to the corresponding rats. The gross appearance, the morphological and electrophysiological changes, the image analysis of axons and motor end-plate were detected at 4, 8, 12 and 16 weeks.

RESULTS

All rates survived to the end of the experiment. The adhesion and edema of allografts in group A and group B were obvious 4 weeks after operation; then adhesion and edema was obvious in group A and were improved in the other groups 8 weeks after operation. Adhesion was observed in groups A and B; no adhesion was observed in groups C and D at 12 weeks. The number of regeneration nerve, the latent, the amplitude, the nerve conduction velocity, the medullary sheath/microm2, the medullary sheath density/microm2 and the image analysis of axons and motor end-plate in groups A and B were significantly lower than those in groups C and D (P < 0.01); and there were no significant differences between groups C and D (P > 0.05). The observation of transmission electron microscope showed that medullated nerve fibers and myelin sheath of groups C and D were thicker than groups A and B, layers of groups C and D were clear.

CONCLUSION

The vitrification solution with 200 mg/L tetramethylpyrazine has protective effect on regeneration of peripheral nerve allografts.

目的

探讨在玻璃化溶液中添加一定浓度的川芎嗪（TMP）对周围神经同种异体移植再生的影响。

方法

选取48只健康清洁级SD雄性大鼠作为供体，96只健康清洁级Wistar雄性大鼠作为受体，所有大鼠均为3月龄，体重200 - 250 g。从供体大鼠取下15 mm长的坐骨神经段，然后根据含TMP的玻璃化溶液将其随机分为4组。A组不使用TMP作为对照组；B组、C组和D组分别使用100 mg/L、200 mg/L和400 mg/L的TMP。然后将其在-196℃冷冻保存3周。在受体大鼠的坐骨神经上制造10 mm长的神经缺损。复温后，将同种异体移植物移植到相应大鼠体内。在4、8、12和16周时检测大体外观、形态学和电生理变化、轴突和运动终板的图像分析。

结果

所有大鼠均存活至实验结束。术后4周时，A组和B组同种异体移植物的粘连和水肿明显；术后8周时，A组粘连和水肿仍明显，其他组有所改善。术后12周时，A组和B组观察到粘连；C组和D组未观察到粘连。A组和B组的再生神经数量、潜伏期、波幅、神经传导速度、髓鞘/μm²、髓鞘密度/μm²以及轴突和运动终板的图像分析均显著低于C组和D组（P < 0.01）；C组和D组之间无显著差异（P > 0.05）。透射电子显微镜观察显示，C组和D组的有髓神经纤维和髓鞘比A组和B组厚，C组和D组的层数清晰。