[龙舌兰TNT对愈伤组织细胞及完整植株细胞影响的超微结构研究]

[Ultrastructural study of TNT effect on the callus cells and the cells of intact plants of Yucca gloriosa L].

作者信息

Gogoberidze M, Zaalishvili G, Ramishvili M, Gogava M, Chelidze N

出版信息

Tsitol Genet. 2009 Jan-Feb;43(1):23-7.

PMID:19663311

Abstract

Intracellular distribution of assimilated 2,4,6-trinitrotoluene (TNT) in callus cells, flower buds and leaves of intact Yucca gloriosa L. plants using electron microscope radioautography. The radiotracer was detected in vacuoles, plastids, mitochondrion, endoplasmic reticulum and cytoplasm. It was found that in dedifferentiated callus cells TNT was incorporated in the vacuoles in greater quantities in comparison with the cells of intact plant. Correspondingly the ultrastructural integrity of the dedifferentiated cells is less damaged.

摘要

利用电子显微镜放射自显影技术研究同化的2,4,6-三硝基甲苯（TNT）在完整凤尾兰植株愈伤组织细胞、花芽和叶片中的细胞内分布。在液泡、质体、线粒体、内质网和细胞质中检测到放射性示踪剂。结果发现，与完整植株的细胞相比，去分化愈伤组织细胞中TNT更多地被整合到液泡中。相应地，去分化细胞的超微结构完整性受损较小。