[红平红球菌EK-1中氧化乙醇和乙醛的脱氢酶]

[Dehydrogenases oxidizing ethanol and acetaldehide in Rhodococcus erythropolis EK-1].

作者信息

Pirog T P, Korzh Iu V, Shevchuk T A

出版信息

Mikrobiol Z. 2009 Jan-Feb;71(1):34-41.

PMID:19663325

Abstract

Four types of alcohol- and acetaldehyde dehydrogenases were found in the cells of strain Rhodococcus erythropolis EK1 grown on ethanol. They are as follows: NAD-, NADP-, pyroquinoline quinone (PQQ)- and 4-nitroso-N,N-dimethyl aniline (NDMA)-dependent enzymes. Activity of NAD- and NADP+ -dependent alcohol dehydrogenases, as well as PQQ and NDMA-dependent acetaldehyde dehydrogenases was low and made up 3-11 nmol x min(-1) x mg(-1) of protein. Ethanol oxidation in the given strain is realized by NDMA-dependent alcohol dehydrogenase, which activity reached its maximum value (up to 15 nmol x min(-1) x mg(-1) of protein) in the early exponential growth phase. Acetaldehyde is oxidized with participation of NAD and NADP-dependent dehydrogenases with optimum pH 9.0-9.5. The results obtained are a basis for development of approaches to intensification of surfactants synthesis by Rhodococcus erythropolis EK-1 strain.

摘要

在以乙醇为生长底物的红平红球菌EK1菌株细胞中发现了四种类型的乙醇脱氢酶和乙醛脱氢酶。它们分别如下：依赖烟酰胺腺嘌呤二核苷酸（NAD）、烟酰胺腺嘌呤二核苷酸磷酸（NADP）、吡咯喹啉醌（PQQ）和4-亚硝基-N，N-二甲基苯胺（NDMA）的酶。依赖NAD和NADP的乙醇脱氢酶以及依赖PQQ和NDMA的乙醛脱氢酶活性较低，蛋白质含量为3 - 11 nmol·min⁻¹·mg⁻¹。给定菌株中的乙醇氧化是通过依赖NDMA的乙醇脱氢酶实现的，该酶活性在指数生长早期阶段达到最大值（高达15 nmol·min⁻¹·mg⁻¹蛋白质）。乙醛在依赖NAD和NADP的脱氢酶参与下被氧化，最适pH为9.0 - 9.5。所得结果为开发强化红平红球菌EK - 1菌株合成表面活性剂方法奠定了基础。