Gregory R L, Kindle J C, Hobbs L C, VanTo T, Malmstrom H S
Department of Oral Biology, Emory University, Atlanta, Georgia 30322.
Hum Antibodies Hybridomas. 1990;1(3):126-31.
Previously, we reported that smokeless tobacco users have significantly higher levels of immunoglobulin A and J chain in whole saliva than non-tobacco users. Because there was no difference in levels of secretory component between the two groups, the proportion of secretory component/immunoglobulin A was significantly lower in users than non-users. There was no significant difference in antibody function. In the present study, we examined immunoglobulin A from whole saliva of users and non-users to determine the effect of smokeless tobacco on the ability of secretory component to bind to immunoglobulin A containing J chain. Whole saliva was passed over an affinity chromatography filter unit coupled with anti-alpha heavy chain-specific antibody followed by passage over a molecular sieve high-performance liquid chromatography column. Peaks were collected and examined for immunoglobulin A, J chain and secretory component by enzyme-linked immunosorbent assay. Saliva from users had three significantly larger peaks (3-4 fold) at 280 nm than non-users, confirming the presence of a higher concentration of immunoglobulin A in users. The secretory component/J chain and secretory component/immunoglobulin A ratios for the largest peak were significantly less in users. This indicates that smokeless tobacco has an effect on the ability of secretory component to bind to immunoglobulin A without a loss in antibody function. This may occur either prior to immunoglobulin A/J chain binding to secretory component receptors on secretory epithelial cells or internal to the epithelial cells. These studies provide further evidence for the role of secretory component in mucosal immunity.
此前，我们报道过，与非烟草使用者相比，无烟烟草使用者全唾液中的免疫球蛋白A和J链水平显著更高。由于两组间分泌成分水平无差异，使用者中分泌成分/免疫球蛋白A的比例显著低于非使用者。抗体功能方面无显著差异。在本研究中，我们检测了使用者和非使用者全唾液中的免疫球蛋白A，以确定无烟烟草对分泌成分与含J链免疫球蛋白A结合能力的影响。全唾液先通过与抗α重链特异性抗体偶联的亲和层析过滤单元，然后再通过分子筛高效液相色谱柱。收集各峰，并通过酶联免疫吸附测定法检测免疫球蛋白A、J链和分泌成分。使用者的唾液在280 nm处有三个显著更大的峰（3 - 4倍），高于非使用者，证实使用者中免疫球蛋白A浓度更高。使用者中最大峰的分泌成分/J链和分泌成分/免疫球蛋白A比值显著更低。这表明无烟烟草对分泌成分与免疫球蛋白A的结合能力有影响，但抗体功能未丧失。这种情况可能发生在免疫球蛋白A/J链与分泌上皮细胞上的分泌成分受体结合之前，或者发生在上皮细胞内部。这些研究为分泌成分在黏膜免疫中的作用提供了进一步证据。
