The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, 216 Tiansheng Rd., Beibei District, Chongqing 400716, China.
Tissue Cell. 2010 Feb;42(1):42-6. doi: 10.1016/j.tice.2009.07.002. Epub 2009 Aug 7.
A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line.
从 21-872nlw 品种的 3 日龄第四龄期家蚕幼虫的卵巢组织中，通过在 Grace 培养基中进行原代培养，添加 20%胎牛血清（FBS），建立了细胞系 BmN-SWU1。该细胞系主要由短梭形细胞和圆形细胞组成。具有染色体数 2n=56 的细胞频率为 80.5%；因此，该细胞系被认为是二倍体细胞系。在第 45 代的倍增时间（PDT）为 57.7h。该细胞系对家蚕核多角体病毒（BmNPV）敏感，在细胞密度为 10(5)个细胞/ml 时，TCID(50)的中位数为 16.3 OBs/ml。该细胞系中绿色荧光蛋白（GFP）基因的瞬时表达效率为 54.8%。我们使用 BmN-SWU1 细胞系来选择和建立 GFP 转基因细胞系。
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