Sharoyan S G, Shaljian A A, Nalbandyan R M, Buniatian H C
Biochim Biophys Acta. 1977 Aug 23;493(2):478-87. doi: 10.1016/0005-2795(77)90204-5.
The procedure for the isolation of two water soluble copper-containing proteins from the white and gray matter of bovine brain is described. One of the proteins, cerebrocuprein I, is superoxide dismutase; and three molecular forms of this enzyme are to be found in brain. The other protein present in gray and white matter is devoid of superoxide dismutase and amine oxidase activities. The amino acid composition, molecular weight, isoelectric point and copper content of this protein were determined. The effect of some agents, pH and thermal treatment of the optical and EPR spectra of the protein were also studied. The copper of the protein may be removed and the holoprotein reconstituted again from apoprotein and copper. The results obtained led to the conclusion that in brain a new copper protein is discovered, which is named neurocuprein.
本文描述了从牛脑白质和灰质中分离两种水溶性含铜蛋白的方法。其中一种蛋白质，脑铜蛋白I，是超氧化物歧化酶；在脑中可发现该酶的三种分子形式。存在于灰质和白质中的另一种蛋白质缺乏超氧化物歧化酶和胺氧化酶活性。测定了该蛋白质的氨基酸组成、分子量、等电点和铜含量。还研究了某些试剂、pH值和热处理对该蛋白质的光学和电子顺磁共振光谱的影响。该蛋白质中的铜可以被去除，并且脱辅基蛋白和铜可以再次重构全蛋白。所得结果得出结论，在脑中发现了一种新的铜蛋白，命名为神经铜蛋白。
